Robert J. Hohman, Ph.D.
Chief, Research Technologies BranchChief, Protein Chemistry Section, RTB
The Protein Chemistry Section enables intramural NIAID investigators to use state-of-the-art applications in mass spectrometry, bioinformatics, peptide synthesis and analysis, and protein sequencing in their research programs.
Mass spectrometry provides extensive support in separation, analysis, and characterization and quantitation of peptides and proteins. Our primary work involves bottom-up proteomics using a state-of-the-art high mass accuracy instrument.
Bioinformatics for mass spectrometry performs both label-free and labeled quantitation methods in addition to routine parsimony analysis.
Peptide synthesis and analysis provides synthetic peptides, peptide-carrier protein conjugates, peptide purification, and quality control analyses. In addition, our staff is available for advice and consultations. In cooperation with protein sequencing and mass spectrometry, extensive support is available for expert analysis and characterization of peptides, proteins, and small bio-molecules.
Protein sequencing enables intramural NIAID investigators to use de novo Edman protein sequencing, using ABI Procise protein sequencers. We offer N-terminal sequence analyses that provide direct chemical sequences for quality control of known proteins or identification of proteins not presently in any database.
Our facility is equipped with specialized instrumentation devoted to the science of protein chemistry, which requires answering biologically relevant questions by examining the structure of proteins at the molecular level.
Carl H. Hammer, Ph.D.Glenn Nardone, Ph.D.Jan Lukszo, Ph.D.L. Renee Olano, Ph.DMark K. Garfield, M.S.Ming Zhao, Ph.D.Raynaldo Martin, B.S.
Carl H. Hammer, Ph.D., Mass SpectrometryL. Renee Olano, Ph.D., BioinformaticsJan Lukszo, Ph.D., Peptide Synthesis and Analysis Mark K. Garfield, M.S., Protein SequencingGlenn Nardone, Ph.D., Assay DevelopmentMing Zhao, Ph.D., Protein Separation
Last Updated October 31, 2012
Last Reviewed October 31, 2012