Comparative Medicine Branch

Jorge Sztein, D.V.M., PhD

Chief Assisted Reproduction Technologies and Cryopreservation Unit

Dr. Sztein obtained his DVM and PhD from the School of Veterinary Sciences, University of La Plata, Buenos Aires, Argentina. He first did mouse embryo transfers during his second year of veterinary school (1976) and assisted in the college’s laboratory animal facility until graduation. Since graduating he has been in charge of the laboratory animal facility of the National Academy of Sciences, Buenos Aires; worked in cryopreservation and transgenic mouse production for the NEI under a Fogarty scholarship; produced the first transgenic rat at the NIH campus; worked at the Jackson Laboratories (JAX) as a staff scientist doing research in cryobiology, which resulted in the JAX’s first approaches to mouse sperm and ovary cryopreservation; been involved in the creation of EMMA; been a faculty member for JAX cryo-preservation courses; worked for NCI Frederick; and returned to NEI to be in charge of their cryopreservation and colony management program. He joined the CMB- NIAID in July 2008 as Chief, Assisted Reproduction Technology including Cryopreservation Unit (ARTiC).

Major Areas of Research

Cryopreservation and assisted reproduction.
In vitro fertilization
Embryo micromanipulation
Genetics of the inbred mice male fertility

Selected Publications

Sztein J; Vasudevan K, Raber J. Refinements in the Cryopreservation of the mouse ovary. JAALAS 09-00127.R2. Accepted. January 12, 2010

Vasudevan K, Raber J, Sztein J. Fertility Comparison between Wild type and Transgenic mice by In Vitro Fertilization. Transgenic Research. 2009. DOI: 10.1007/s11248-009-9336-2. July 2009

Khon, M.J; Sztein, J.; Yagi, R.; DePamphilis, M.L.; Kaneko, K.J; The acrosomal protein Dickkopf-like 1 (DKKL1) facilitates sperm penetration of the zona pellucida. Fertility and Sterility. July 2009.

N. Tserentsoodol, J. Sztein, M. Campos, S. Fliesler, N. Gordiyenko, Robert N. Fariss, JW Lee, and I. Rodriguez Uptake of cholesterol by the retina occurs primarily via an LDL-receptor mediated process.. Mol Vis 2006; 12:1306-1318

Gordiyenko N, Campos M, Lee JW, Fariss RN, Sztein J, Rodriguez IR. RPE Cells Internalize Low-Density Lipoprotein (LDL) and Oxidized LDL (oxLDL) in Large Quantities In Vitro and In Vivo. Invest Ophthalmol Vis Sci. 2004 Aug;45(8):2822-9

Awasthi P, French C, Sztein J, Bedigian R, Sharp J, Lloyd K. Frozen sperm as an alternative to Shipping live mice. Report. Contemp. Topics. Vol 42, 5. September 2003

Sztein JM, Noble K, Farley JS, Mobraaten LE. 2001. Comparison of permeating and nonpermeating cryoprotectants for mouse sperm cryopreservation. Cryobiology 41, 28-39.

Sztein JM, Farley JS, Mobraaten LE. 2000. In Vitro Fertilization with Cryopreserved Inbred Mouse Sperm. Biol. Reprod. 63, 1774-1780.

Sztein JM, O'Brien MJ, Farley JS, Mobraaten LE, Eppig JJ. 2000. Rescue of oocytes from antral follicles of cryopreserved mouse ovaries: competence to undergo maturation, embryogenesis, and development to term. Hum Reprod, 15(3): 567-571.

Books Chapters:

Sztein JM, Kastenmayer R, Perdue K. Rederivation of mice. 2009. ISTT manual. Accepted. In press.

Sztein JM. Spanish Translation of the “Manual for Sperm Cryopreservation”, The Jackson Laboratories. November 2009.