Laboratory of Viral Diseases

Genetic Engineering Section

Research Videos

Ward, B. M., A. S. Weisberg, B. Moss. 2003. Mapping and Functional Analysis of Interaction Sites within the Cytoplasmic Domains of the Vaccinia Virus A33R and A36R Envelope Proteins. Journal of Virology. 77: 4113-4126.

Figure 8

• vB5R-GFP (QuickTime)
• vB5R-GFP/ΔA36R (QuickTime)
• vA36R^1-123-V5 (QuickTime)
• vA36R^1-111-V5 (QuickTime)
• vA36R^1-93-V5 (QuickTime)
• vA36R^1-80-V5 (QuickTime)
• vA33R^Δ5-40 (QuickTime)

Moss, B. and B. M. Ward. 2001. High-sped mass transit for poxviruses on microtubules. Nature Cell Biology. 3:E245-6

• Visualization of IEV acquiring an actin tail. (QuickTime)

Ward, B. M., and B. Moss. 2001. Vaccinia virus intracellular movement is associated with microtubules and independent of actin tails. Journal of Virology. 75:11651-63

#1. Visualization of actin tail formation by complementation of vΔA36R:

• vΔA36R & pSC11-A36R (Positive Control) (QuickTime)
• vΔA36R & pSC11 (Negative Control) (QuickTime)
• vΔA36R & pSC11-A36R-YdF (QuickTime)

#2. Visualization of IEV using digital video microscopy.

• Figure 9 (QuickTime)

#3. Association of extracellular virus with actin tails.

• Figure 10 Rotation (QuickTime)
• Figure 10 Slices (QuickTime)

#4. Visualization of actin tails formed by vaccinia virus WR.

• WR, GFP-Actin (QuickTime)

Ward, B. M., and B. Moss. 2001. Visualization of intracellular movement of vaccinia virus virions containing a green fluorescent protein-B5R membrane protein chimera. J. Virol. 75:4802-4813

Figure 5. Visualization of IEV by time-lapse confocal microscopy.

• Figure 5A (Quicktime, Real Player)
• Figure 5B (Quicktime, Real Player)
• Figure 5C (Quicktime, Real Player)
• Figure 5D (Quicktime, Real Player)

Figure 7. Visualization of IEV acquiring an actin tail.

• Figure 7GFP (Quicktime, Real Player)
• Figure 7TLD (Quicktime, Real Player)

Figure 8. Effect of nocodazole on virion movement.

• Figure 8A (Quicktime, Real Player)
• Figure 8B (Quicktime, Real Player)
• Figure 8C (Quicktime, Real Player)
• Figure 8D (Quicktime, Real Player)

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