To use this software you must have a local account set up on the confocal computer. If you do not have one, please see Juraj Kabat or Owen Schwartz.
The confocal software saves files in a database system, with a file extension .lei. There also is a .txt file saved to allow you to view the file parameters from outside the Leica software.
The Leica DMIRBE microscope uses its own internal program to control the objective turret and focus motor. All of the objectives, Wollaston prisms, and phase rings have been programmed in already. Please do not change these settings.
All of the objectives to be used in the confocal imaging mode are oil immersion objectives and have short working distances. Place a small drop of oil on the objective before putting your slide on the stage. Different brands of immersion oil are not miscible and will cause serious imaging problems if mixed. If in doubt, be sure and clean your slides of any old immersion oil before starting.
If you are planning to open and adjust your images in Adobe Photoshop, the following initial procedures are recommended:
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Last Updated April 05, 2007