This protocol describes the preparation of a coverslip mounting solution called Mowiol. Mowiol (Mowiol 4.88, Calbiochem catalog number 475904) is a solution of polyvinyl alcohol. This solution normally hardens overnight after slide preparation, and does not require the coverslips to be sealed with nail polish. The addition of PPD (see below) is recommended to reduce bleaching of fluorescent probes.
Note: Do not use so much mounting solution that the coverslips are floating. Normally, 15 – 20 ul is sufficient for a 22 x 22 mm coverslip. 22 x 50 mm coverslips require about 40 – 50 ul.
PPD = p-phenylenediamine (1,4-Benzenediamine hydrochloride, Sigma # P1519)
Note: This compound is carcinogenic and should be handled with care.
Reference: Vanes K, Brandt P. Retardation of immunofluorescence during microscopy. J Histochem Cythochem. 1985. 33:755-61.
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Last Updated April 05, 2007