Cells pass through a 70µm nozzle tip on the instrument, so a single cell suspension is essential. Large cell clumps will settle out of a cell preparation in 10 minutes at 1 g. Please filter cells through a 50µm nylon mesh (PGC Scientific Corp.) after all staining procedures to remove clumps and debris. Clumps can clog the instrument fluidics and in some cases divert the waste stream into the collection tube, thus contaminating the sorted cells and ruining the entire experiment up to that point.
You will need two types of media:
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Last Updated February 15, 2007