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General Guidelines
Because these resources and reagents are limited in quantity, a request process
for scientists to access them has been established. Requestors
interested in obtaining reagents submit a brief proposal to NIAID's Pathogen Functional Genomics Resource Center (PFGRC) describing their research plans for using the reagents. See the Request Process section of this Web site for more information.
Applications will be accepted and reviewed on a continuous basis.
NIAID intends to support investigators requesting reagents for both exploratory/developmental
research projects and established research projects. Microarrays and
related reagents are provided for research purposes only; using them
for commercial purposes is prohibited. In publications of studies using
microarrays supplied by PFGRC, investigators are requested to acknowledge
NIAID/PFGRC as the source of reagents.
- DNA oligonucleotide glass slide microarrays
available now! PFGRC
will provide up to 150 microarrays for a given organism per
request.
Please note: PFGRC will be accepting microarray proposals through 2008 on an ongoing basis.
Microarrays
are available for the following organisms (arrays added in 2007 are in bold):
- Aspergillus fumigatus
- Aspergillus nidulans
- Bacillus anthracis
- Burkholderia
- Campylobacter jejuni (New - Nov. 11, 2007)
- Candida albicans
- Chlamydia family
- Clostridium botulinum
- Coronavirus
- Escherichia coli (Diarrheagenic) (coming soon)
- Francisella tularensis
- Giardia lamblia
- Group B Streptococcus agalactiae
- Haemophilus influenza
- Helicobacter pylori
- Listeria monocytogenes
- Mycobacterium smegmatis
- Mycobacterium tuberculosis
- Neisseria gonorrhoeae
- Plasmodium falciparum
- Plasmodium vivax
(New - Nov. 11, 2007)
- Pseudomonas aeruginosa
- Rickettsia prowazekii
- Salmonella typhimurium
- Staphylococcus aureus
- Streptococcus pneumoniae
- Streptococcus pyogenes
- Trypanosoma brucei
- Trypanosoma cruzi
- Vibrio cholerae
- Vibrio parahaemolyticus (New - Nov. 11, 2007)
- Vibrio vulnificus (New - Nov. 11, 2007)
- Yersinia pestis
NIDCR also offers five Oral Microbe Microarrays:
The National Institute of Dental and Craniofacial Diseases (NIDCR) is pleased to announce the continuation of an initiative to provide no-cost, oligonucleotide genomic DNA microarray slides ("chips") for oral bacteria. This initiative is a collaborative effort between the NIDCR and the National Institute of Allergy and Infectious Diseases (NIAID) Pathogen Functional Genomics Resource Center (PFGRC) located at the J. Craig Venter Institute (JCVI). The NIAID PFGRC is a centralized facility to produce and distribute high quality oligonucleotide glass microarrays to the scientific community. The PFGRC serves as an important resource to infectious diseases researchers wishing to investigate the genomics of human pathogens and invertebrate vectors of diseases, including now oral pathogens.
Currently, requests for the following oral bacteria genomic microarrays are now being accepted:
Please visit their link: http://www.nidcr.nih.gov/Research/Extramural/IntegrativeBiology/Microbiology/Microarray/default.htm to find out how to request these arrays.
Invitrogen Gateway® Entry Clones are now available for distribution. NIAID's Pathogen Functional Genomics Resource Center (PFGRC) at The Institute for Genomic Research (TIGR) announces the availability of clone sets from select microbial pathogens cloned into Invitrogen's Gateway® Cloning Technology. Clone sets are now available from Bacillus anthracis, Francisella tularensis, Staphylococcus aureus, Streptococcus pneumoniae, Yersinia pestis, and Vibrio cholerae. In addition, the PFGRC is providing, at no cost to investigators, a custom clone ordering process that will allow investigators to specify and order up to 96 Invitrogen Gateway® Entry Clones at a time from a select group of microbial pathogens. A complete description of the available Invitrogen Gateway® Entry Clones and the ordering process can be found at the J. Craig Venter Institute PFGRC Web site. Please check the site for updates regarding the inventory of available clone sets, as well as additions to the custom ordering catalogue.
SARS-CoV Gateway® Clone Set available. NIAID's PFGRC has constructed
and is making available a clone set called the SARS-CoV Gateway® Clone
Set. This set consists of twenty seven (27) clones containing
ORFs based on the Genbank accession number NC_004718.3. Each clone
in the set is clearly identified by its accession. The clones are
constructed in the vector pDONR 221 (Invitrogen, Inc), and these
'entry clones' are constructed such that in-frame expression constructs
can
be readily produced through the use of Invitrogen's Gateway® Cloning
Technology. Investigators who are unfamiliar with Invitrogen's Gateway®
Cloning Technology can consult the Invitrogen
educational Web site for further information. Information about
obtaining this SARS-CoV Gateway® Clone Set and specific data pertaining
to each clone is available on the J. Craig Venter Institute PFGRC Web site.
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