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Leading research to understand, treat, and prevent infectious, immunologic, and allergic diseases
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Infectious Disease Pathogenesis Section
Comparative Medicine Branch

Major Areas of Research

  • Pathogenesis of infectious disease
  • Nonhuman primate pathology and immunohistochemistry

Program Description

The Infectious Disease Pathogenesis Section provides molecular and necropsy support to NIAID investigators and programs that conduct research involving animals. Our veterinary pathologists have expertise in the gross and microscopic evaluation of both spontaneous and experimental disease and provide guidance on the design, conduct, interpretation, and publication of studies involves laboratory animals.

CD45R/B220-labeled B-lymphocytes (red), CD3-labeled T-lymphocytes (green), and DAPI (blue) CD45R/B220-labeled B-lymphocytes (red), Iba1-labeled macrophages (green), and DAPI (blue)
Double-Label Immunofluorescence of a Lymphoid Follicle
Left: CD45R/B220-labeled B-lymphocytes (red), CD3-labeled T-lymphocytes (green), and DAPI (blue). Right: CD45R/B220-labeled B-lymphocytes (red), Iba1-labeled macrophages (green), and DAPI (blue).

Services Offered

Immunohistochemistry (IHC) and Immunofluorescence (IF)

  • IHC and IF on formalin-fixed, paraffin-embedded sections
  • Many commercially available antibodies optimized for use in rodents and nonhuman primates
  • Protocol development for new antibodies, including antigen retrieval
  • Microtome sectioning onto charged slides
  • Detection methods: Standard ABC method (peroxidase and alkaline phosphatase), fluorescence (single and multi-label protocols)
  • Archived paraffin blocks for positive or negative control tissues are available


  • Trimming of fixed tissues into cassettes and submission to Histoserv, Inc. for processing, embedding, and hematoxylin and eosin (H&E) staining
  • Microtomy of paraffin sections

Necropsy & Pathology

  • Necropsy support either on-site at NIAID facility or by submission to the lab (rodents only)
  • Evaluation and scoring of H&E-stained sections
  • Interpretation of IHC and IF data
  • Slide scanning with Aperio ScanScope XT
  • Photomicroscopy

Publication Support

  • Assist with manuscript preparation, including production of figures, data tables, and production of publication-quality images
  • Writing of accompanying material and methods and results sections

Contact Us

Ian Moore, D.V.M., Ph.D., Veterinary Pathologist
Mahnaz Minai, Histotechnologist
Kevin Bock, Histotechnologist

Contact Dr. Ian Moore by email or phone with inquiries or questions regarding current or new projects.

Hours of Operation

7:00 a.m. – 3:30 p.m., Monday–Friday
Closed on all federal holidays


Twinbrook III, 2nd Floor
Office: Room 2W-01
Lab: Room 2W-03

Mailing Address

12735 Twinbrook Parkway, Rm 2W-03
Rockville, MD 20892-8134

Selected Publications

Boonnak K, Vogel L, Orandle M, Zimmerman D, Talor E, Subbarao K. Antigen-activated dendritic cells ameliorate influenza A infections. J Clin Invest. 2013 Jul 1;123(7):2850-61.

Via LE, Weiner DM, Schimel D, Lin PL, Dayao E, Tankersley SL, Cai Y, Coleman MT, Tomko J, Paripati P, Orandle M, Kastenmayer RJ, Tartakovsky M, Rosenthal A, Portevin D, Eum SY, Lahouar S, Gagneux S, Young DB, Flynn JL, Barry CE 3rd. Differential virulence and disease progression following Mycobacterium tuberculosis complex infection of the common marmoset (Callithrix jacchus). Infect Immun. 2013 Aug;81(8):2909-19.

Waisberg M, Lin CK, Huang CY, Pena M, Orandle M, Bolland S, Pierce SK. The impact of genetic susceptibility to systemic lupus erythematosus on placental malaria in mice. PLoS One. 2013 May 10;8(5):e62820.

Cover of the American Journal of Pathology, Cellular and Molecular Biology of Disease
Reprinted from Am J Pathol 2012, 180:1049-58 with permission from the American Society for Investigative Pathology. Cover image by Dr. Marlene Orandle. Click to see larger image.

Yi L, Liu Q, Orandle MS, Sadiq-Ali S, Koontz SM, Choi U, Torres-Velez FJ, Jackson SH. p47(phox) directs murine macrophage cell fate decisions. Am J Pathol. 2012 Mar;180(3):1049-58.

Suguitan AL Jr, Matsuoka Y, Lau YF, Santos CP, Vogel L, Cheng LI, Orandle M, Subbarao K. The multibasic cleavage site of the hemagglutinin of highly pathogenic A/Vietnam/1203/2004 (H5N1) avian influenza virus acts as a virulence factor in a host-specific manner in mammals. J Virol. 2012 Mar;86(5):2706-14.

Shulzhenko N, Morgun A, Hsiao W, Battle M, Yao M, Gavrilova O, Orandle M, Mayer L, Macpherson AJ, McCoy KD, Fraser-Liggett C, Matzinger P. Crosstalk between B lymphocytes, microbiota and the intestinal epithelium governs immunity versus metabolism in the gut. Nat Med. 2011 Nov 20;17(12):1585-93.


Photo of Olympus BX-51 microscope with DP-70 color camera Photo of Boeckeler MR-3rotary microtome
Left: Olympus BX-51 microscope with DP-70 color camera; Right: Boeckeler MR-3 rotary microtome
Photo of Aperio ScanScope XT Photo of TBS SHUR/Mark cassette and slide labeling instrument
Left: Aperio ScanScopeXT; Right: TBS SHUR/Mark cassette and slide labeling instrument

Last Updated October 27, 2014