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Contact Info

Dean D. Metcalfe, M.D.
Building 10, Room 11C207
10 Center Drive, MSC 1881
Bethesda, MD 20892-1881
Phone: 301-496-2165
Fax: 301-480-8384
dmetcalfe@niaid.nih.gov

Research Feature

A pediatric patient performing IOS. The patient is using a nose-clip and making a tight seal with his lips at the mouthpiece of the IOS device. The results of IOS testing are displayed on the computer monitor. Read more about it.

Laboratory of Allergic Diseases

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Mast Cell Biology Section

Dean D. Metcalfe, M.D., Chief


Description of Research Program

The mast cell is the focus of the Mast Cell Biology Section (MCBS) research effort. This multifunctional inflammatory cell is involved in both innate and acquired immunity and plays a central role in the induction of allergic inflammation. An integrated program investigating mast cell biology includes studies into the growth and differentiation of mast cells, mast-cell signal transduction, and the products generated by mast cells that lead to disease. The MCBS program emphasizes basic research that may be translated into the clinic, where protocols include studies on the pathogenesis of urticaria, anaphylaxis, and mastocytosis. Research efforts have contributed to the identification of mutations in clonal mast cell disorders, understanding signaling through KIT and the high affinity IgE receptors, and how alterations in the control of mast cell mediator production affect human disease.

Future Efforts

  • Identification of mutations and polymorphisms in human disease that affect the mast cell compartment
  • Characterization of key signaling pathways in human mast cells that control mast cell responses
  • Application of this information to the diagnosis and treatment of anaphylaxis and other allergic and immunologic diseases
Front row (left to right): Melody Carter,Nevenka Medic, Geethani Bandara, Mi-Yeon Jung, Avanti Desai, Arnold Kirshenbaum, Eunice Chan, Yun Bai
Back row (left to right): Daniel Smrz, Alasdair Gilfillan, Hirsh Komarow, Todd Wilson, Dean Metcalfe, Section Chief, Frank Lichtenberger, Stephen Music, Glenn Cruse
Front row (left to right): Melody Carter, Nevenka Medic, Geethani Bandara, Mi-Yeon Jung, Avanti Desai, Arnold Kirshenbaum, Eunice Chan, Yun Bai
Back row (left to right): Daniel Smrz, Alasdair Gilfillan, Hirsh Komarow, Dean Metcalfe, Section Chief, Frank Lichtenberger, Stephen Music, Glenn Cruse

Selected Recent Publications

To view a complete listing, visit PubMed.

Wilson TM, Maric I, Shukla J, Brown M, Simakova O, Fay MP, Kozhich A, Kolbeck R, Metcalfe DD, Nutman TB, Klion AD. Interleukin-5 receptor alpha levels in patients with marked eosinophilia or mastocytosis. J Allergy Clin Immunol. 2011. In press.

Kuehn HS, Jung MY, Beaven MA, Metcalfe DD, Gilfillan AM. Prostaglandin E2 activates and utilizes mTORC2 as a central signaling locus for the regulation of mast cell chemotaxis and mediator release. J Biol Chem. 2011 Jan 7;286(1):391-402.

Kataoka TR, Kumanogoh A, Bandara G, Metcalfe DD, Gilfillan AM. CD72 negatively regulates KIT-mediated responses in human mast cells. J Immunol. 2010 Mar 1;184(5):2468-75.

Metcalfe DD, Peavy RD, Gilfillan AM. Mechanisms of mast cell signaling in anaphylaxis. J Allergy Clin Immunol. 2009 Oct;124(4):639-46; quiz 647-8.

Iwaki S, Spicka J, Tkaczyk C, Jensen BM, Furumoto Y, Charles N, Kovarova M, Rivera J, Horejsi V, Metcalfe DD, Gilfillan AM. Kit- and Fc epsilon RI-induced differential phosphorylation of the transmembrane adaptor molecule NTAL/LAB/LAT2 allows flexibility in its scaffolding function in mast cells. Cell Signal. 2008 Jan;20(1):195-205.

Lahortiga I, Akin C, Cools J, Wilson TM, Mentens N, Arthur DC, Maric I, Noel P, Kocabas C, Marynen P, Lessin LS, Wlodarska I, Robyn J, Metcalfe DD. Activity of imatinib in systemic mastocytosis with chronic basophilic leukemia and PRKG2-PDGFRB fusion. Haematologica. 2008 Jan;93(1):49-56.

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Last Updated December 21, 2012