David H. Margulies, M.D., Ph.D.
Chief, Molecular Biology Section, LI
The Molecular Biology Section (MBS) is directed toward a precise molecular understanding of critical interactions that initiate, control, and perpetuate the immune response. To this aim, the laboratory focuses on
In recent years, the major accomplishments of MBS have included determination of the structure and function of the CD8 alpha beta T-cell coreceptor in complex with its MHC-I ligand, studies of the mechanism of peptide loading of the MHC-I molecule, and studies of the structure and function of viral immunoevasins. These last advances include the determination of the structure of the complex of the mouse cytomegalovirus MHC-I-like molecule m152 in complex with its ligand RAE1.
Using purified MHC molecules, viral immunoevasins, TCR, and NK receptors, we have learned the thresholds of affinity that allow such cell surface receptors to activate or inhibit effector-cell function. Based on X-ray crystallographic studies of these components and of their molecular complexes, we now understand in molecular detail the mechanism of peptide loading of MHC-I molecules, the basis of molecular recognition of TCR for MHC-I and MHC-II molecules, and the structural basis for interaction of T-cell coreceptors with MHC molecules. X-ray structures of MHC-II/peptide complexes that are crucial to the development of autoimmunity provide insight into the molecular basis of autoimmunity.
Our recent studies of viral immunoevasins reveal the structural evolution of viral resistance to recognition by the immune system and provide opportunities for developing strategies to counter such viral evasion. In addition, our broad understanding of the structure-function relationships of MHC molecules has permitted us to explore the relationship of MHC-I structure to HLA-linked drug-induced hypersensitivity syndromes. We are currently developing mouse model systems to allow further exploration of the molecular and cellular basis of such HLA-linked syndromes.
Dr. Margulies received an A.B. from Columbia University in 1971. In 1978, he earned his M.D. and Ph.D. from the Albert Einstein College of Medicine. From 1978 to 1980, he served as a resident in medicine at Columbia/Presbyterian Medical Center. From 1980 to 1983, he worked as a research associate in the Laboratory of Molecular Genetics at the National Institute of Child Health and Human Development. From 1983 to 1987, he was an investigator in the Laboratory of Immunology. In 1987, he became a senior investigator and, since 1989, has been chief of the Molecular Biology Section. Since 2008, he has been a member of the Senior Biomedical Research Service.
Phi Beta Kappa; U.S. Public Health Service Commendation Medal, 1987; Outstanding Service Medal, 1991; Meritorious Service Medal, 1997; Distinguished Service Medal, 2001; NIH Merit Award, 2009 (Tetramer Facility Team)
Immunology, Structural Biology
Lisa F. Boyd, Rosalene Carey, Michael G. Mage, Nathan May, Giora Morozov, Kannan Natarajan, Mulualem Tilahun
Wang R, Natarajan K, Revilleza MJ, Boyd LF, Zhi L, Zhao H, Robinson H, Margulies DH. Structural basis of mouse cytomegalovirus m152/gp40 interaction with RAE1γ reveals a paradigm for MHC/MHC interaction in immune evasion. Proc Natl Acad Sci U S A. 2012 Dec 18;109(51):E3578-87.
Mage MG, Dolan MA, Wang R, Boyd LF, Revilleza MJ, Robinson H, Natarajan K, Myers NB, Hansen TH, Margulies DH. The peptide-receptive transition state of MHC class I molecules: insight from structure and molecular dynamics. J Immunol. 2012 Aug 1;189(3):1391-9.
Norcross MA, Luo S, Lu L, Boyne MT, Gomarteli M, Rennels AD, Woodcock J, Margulies DH, McMurtrey C, Vernon S, Hildebrand WH, Buchli R. Abacavir induces loading of novel self-peptides into HLA-B*57: 01: an autoimmune model for HLA-associated drug hypersensitivity. AIDS. 2012 Jul 17;26(11):F21-9.
Revilleza MJ, Wang R, Mans J, Hong M, Natarajan K, Margulies DH. How the virus outsmarts the host: function and structure of cytomegalovirus MHC-I-like molecules in the evasion of natural killer cell surveillance. J Biomed Biotechnol. 2011;2011:724607.
Zhi L, Mans J, Paskow MJ, Brown PH, Schuck P, Jonjić S, Natarajan K, Margulies DH. Direct interaction of the mouse cytomegalovirus m152/gp40 immunoevasin with RAE-1 isoforms. Biochemistry. 2010 Mar 23;49(11):2443-53.
Wang R, Natarajan K, Margulies DH. Structural basis of the CD8 alpha beta/MHC class I interaction: focused recognition orients CD8 beta to a T cell proximal position. J Immunol. 2009 Aug 15;183(4):2554-64.
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Last Updated August 19, 2013