Program Description
The Flow Cytometry Section enables intramural NIAID investigators to utilize state-of-the-art flow cytometric analysis and sorting technologies in their research programs. We provide expertise, instrumentation, and analysis. Our staff is accessible for consultation in the design of experiments and in interpretation and analysis of data.
Instrumentation
Our facility is equipped with specialized instrumentation dedicated to the science of flow cytometry, which entails answering biological questions by analysis of multiparameters at the individual cell level.
Instruments include operator-run cell sorters and user-operated analysis machines:
- Sorters: Eight BD FACS Aria II cell sorters (two in BSL-3 containment)
- Analyzers: Three BD LSR II’s (one in BSL-3 containment), one LSR/Fortessa, four FACS Caliburs, one FACS Canto II, one AMNIS Imagestream 100, and one Millipore MagPix.
Custom Antibodies
Larry M. Lantz, Ph.D., Custom Antibodies
We also provide a cost- and time-efficient method of obtaining fluorescent labeled antibodies. Resources are available to all intramural NIAID investigators and include
- Hybridoma expansion
- Antibody purification from ascites, antisera, and hybridoma supernatant
- Coupling of purified antibodies and proteins to various fluorochromes
Research Group
Kevin L. Holmes, Ph.D.
Bishop Hague, Ph.D.
Calvin Eigsti, B.A.
Carol Henry, B.A.
David Stephany, B.S.
Elina Stregevsky, B.S.
Larry M. Lantz, Ph.D.
Mehrnoosh Abshari, M.S.
Roy Wheat, B.S.
Thomas Moyer, B.S.