Skip Navigation

Research Technologies Branch

Skip Content Marketing
  • Share this:
  • submit to facebook
  • Tweet it
  • submit to reddit
  • submit to StumbleUpon
  • submit to Google +

Beth Fischer, M.A.

Photo of Beth Fischer

Chief, RML Microscopy Unit, RTB

Major Areas of Research

See more about these areas in RML Microscopy Techniques and Technologies.

 

Program Description

The Electron Microscopy (EM) Unit is dedicated to providing state-of-the-art technologies to support the ultrastructural imaging needs of NIAID scientists both in the Bethesda, Maryland, area and at the Rocky Mountain Laboratories in Hamilton, Montana.

The facility provides sample preparation and analysis ranging from basic to high resolution structural studies to immuno-localization of selected antigens for a wide array of specimens. A variety of methods, protocols, and equipment are employed to accommodate different preparative and imaging needs. Real-time viewing of samples by video image-sharing is available to NIAID users, enabling scientists at distant locations the ability to view samples simultaneously with microscopy staff.

Research Group

Beth Fischer, M.A.
David Dorward, Ph.D.
Vinod Nair, Ph.D.
Bryan Hansen, B.S.

Photo of RTB Microscopy research group
Left to right: Bryan Hansen, Elizabeth (Beth) Fischer, Vinod Nair, David Dorward

Selected Publications

Chen Z, Fischer ER, Kouiavskaia D, Hansen BT, Ludtke SJ, Bidzhieva B, Makiya M, Agulto L, Purcell RH, Chumakov K. Cross-neutralizing human anti-poliovirus antibodies bind the recognition site for cellular receptor. Proc Natl Acad Sci U S A. 2013 Dec 10;110(50):20242-7. doi: 10.1073/pnas.1320041110. Epub 2013 Nov 25. PMID:24277851

Ngamskulrungroj P, Chang Y, Hansen B, Bugge C, Fischer E, Kwon-Chung KJ. Characterization of the chromosome 4 genes that affect fluconazole-induced disomy formation in Cryptococcus neoformans. PLoS One. 2012;7(3):e33022.

Offerdahl DK, Dorward DW, Hansen BT, Bloom ME. A three-dimensional comparison of tick-borne flavivirus infection in mammalian and tick cell lines. PLoS One. 2012;7(10):e47912

Fischer ER, Hansen BT, Nair V, Hoyt FH, Dorward DW. Scanning electron microscopy. Curr Protoc Microbiol. 2012 May;Chapter 2:Unit 2B.2.

Belov GA, Nair V, Hansen BT, Hoyt FH, Fischer ER, Ehrenfeld E. Complex dynamic development of poliovirus membranous replication complexes. J Virol. 2012 Jan;86(1):302-12.

Cocchiaro JL, Kumar Y, Fischer ER, Hackstadt T, Valdivia RH. Cytoplasmic lipid droplets are translocated into the lumen of the Chlamydia trachomatis parasitophorous vacuole. Proc Natl Acad Sci U S A. 2008 Jul8;105(27):9379-84.

Visit PubMed for a complete list of publications.

Equipment

The RML Microscopy Unit has state-of-the-art microscopy equipment, including two scanning electron microscopes, a high-pressure freezer, high-resolution transmission electron microscopes with 3D tomography capabilities, and more.

Photo of FEI/Titan Krios 300kV TEM
FEI/Titan Krios 300kV TEM
Enables higher resolution for 2- and 3-D imaging of room-temperature or cryo specimens less than or equal to 1 micron.
Credit: NIAID
Photo of Hitachi SU-8000
Hitachi SU-8000, a semi-in-lens 30 kV field emission electron microscope with secondary, backscatter, and STEM detector.
Credit: NIAID
 

Images

Photo of Group a Streptococcus on primary neutrophil
Group A Streptococcus on primary neutrophil.
Credit: NIAID
Photos of Surface antigen labeling of spirochetes prepared by negative staining (A) or thin sectioning (B) viewed by TEM, or by backscatter detection (C) viewed by SEM
Surface antigen labeling of spirochetes prepared by negative staining (A) or thin sectioning (B) viewed by TEM, or by backscatter detection (C) viewed by SEM.
Credit: NIAID
 
After freezing specimens in suspension, regions of interest are determined under low-dose conditions to avoid radiation damage. Ideal imaging takes place in areas with very thin ice, usually where specimens are well-spread
After freezing specimens in suspension, regions of interest are determined under low-dose conditions to avoid radiation damage. Ideal imaging takes place in areas with very thin ice, usually where specimens are well-spread.
Credit: NIAID
 

See more on Flickr.

Last Updated December 17, 2013

Last Reviewed December 10, 2013