Vaccine Research Center (VRC)

Mario Roederer, Ph.D.

Flow Cytometry Core Section

Quantum dots suspended in PBS and placed into the 407nm Violet DPSS laser. The emission shows the wide range of the visible spectrum, which can be measured from this single laser

Description of Research Program

The VRC Flow Cytometry Core Facility contains seven instruments with capabilities of serving a wide range of flow cytometric clinical and research demands. Currently, the laboratory has three FACSCaliburs, which can measure up to 4-colors and these are equipped with a HTS (High Throughput Sampler) device that aids in fast acquisition of specimens from 96 well tray. The FACSDiVa sorter is capable of high-speed, high-end multicolor analysis, measuring 18 parameters. Two LSR II instruments are equipped with HTS units and are capable of measuring 20 parameters. Additionally, the FACSAria is a 20 parameter high-speed sorter located in our BSL-3 facility and is capable of infectious cell sorting. (Please see the Instrumentation section links for more detail).

The VRC Flow Cytometry Core Facility services the research requirements of the VRC investigators as well as the clinical needs of the vaccine trials supported by the VRC. Please contact Stephen Perfetto or Mario Roederer for more information.

VRC Flow Cytometry Core Facility Usage and Policies

Usage within the VRC: All VCR users access electronic sign-up calendars located in the public folder on the Outlook Mail Server for all immunophenotyping procedures. Sorting procedures are scheduled through the VRC core facility personnel only.

Collaboration outside of the VRC: Access to the facility is limited to those who collaborate with VRC investigators. Please contact Mario Roederer for more information.

Instrumentation

FACSCalibur

FACSDiVA

LSR II

FACSAria

General Procedures

Quality Control Procedures
Decontamination Procedure (Aria)
Bio-Containment and Infectious Cell Sorting (Aria)

References

FCC References

Fluorochrome Spectra and Relative Error Contribution

Common Fluorochromes in Flow Cytometry Table (PDF)
Common Fluorochromes Spectra (PDF)
Alexa dyes Spectra (PDF)
Quantum dots Spectra (PDF)

Research Group Members

Mario Roederer, Stephen Perfetto, Richard Nguyen, David Ambrozak.

From Left to Right: Stephen Perfetto, Mario Roederer,
Richard Nguyen, and David Ambrozak.

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