Mass Spectrometry Proteomics specialist, RML Protein Chemistry Unit

Job Announcement Description

The Division of Intramural Research (DIR) is recruiting a mass spectrometry proteomics specialist for the Rocky Mountain Laboratories (RML) Protein Chemistry Unit within the Research Technologies Branch. Mass spectrometry-based proteomics is an important technology in biomedical research and is particularly critical for DIR efforts in immunology, parasitology, infectious disease, allergic disease, and vaccine development. Position goals include providing access to state-of-the art technologies, helping design and run experiments, facilitating data interpretation, and providing results that are consistently high quality. He or she will operate state-of-the-art instrumentation requiring a thorough knowledge of mass spectrometry as it applies to biological applications. The applicant for the Protein Chemistry Unit will have knowledge of current literature and interact with DIR scientists as well as investigators from other National Institutes of Health (NIH) institutes.

The duties of this position are wide-ranging and include the following:

  • Consult with DIR investigators, including meetings prior to the initiation of new proteomics experiments
  • Develop complex acquisition and sample preparation workflows with investigators
  • Configure and maintain high resolution mass spectrometry instrumentation (ThermoScientific)
  • Operate and maintain nano-LC (ThermoScientific)
  • Troubleshoot instruments when problems are encountered
  • Develop technology and collaborate with investigators to facilitate novel applications, including bottom-up labelled and label-free DDA and DIA as well as middle-down and top-down proteomics
  • Perform and analyze SRM/PRM experiments
  • Experience with cross-linking acquisitions and analysis
  • Perform sample preparation including enrichment, digestions, and labeling
  • Process and analyze collected acquisition data using programs including Xcalibur, Proteome Discoverer, Peaks, MaxQuant, and Prosight
  • Train and advise DIR investigators in both sample preparation and analysis
  • Collaborate in preparation of manuscripts for publication
  • Organize mass spectrometry practical tutorials and workshops
  • Acquire quotes and prepare budget requests for new instrumentation
  • Consult with facility managers to design and mass spectrometry spaces


Interested candidates must possess a doctoral degree (Ph.D.) and at least 5 years of related experience post-doctorate. In addition, the candidate must have at least one year of experience in a mass spectrometry core facility working with multiple investigators. The candidate must have extensive experience with bacteria, virus, parasites, disease vector, and other non-mammalian proteomics including sample preparation techniques and analysis design in addition to a working knowledge of mass spectrometry technology and proteomics. The applicant must be proficient with Xcalibur for instrument control and acquisition setup as well as Proteome Discoverer, Peaks, Prosight, and MaxQuant for analysis. The candidate must have experience with training and collaborating on proteomic experiment study design.

To Apply

Interested candidates should send their curriculum vitae (CV), bibliography, and a one- to two-page statement on their background and interests in the position to by October 13, 2021.

HHS, NIH, and NIAID are equal opportunity employers dedicated to diversity, equity, inclusion, and accessibility.  


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