3D Tomography and High-Resolution TEM

The EM unit has an FEI 120 kV Tecnai Spirit Biotwin and a FEI 300 kV Titan Krios TEM for 2D and cryo-tomographic capabilities to provide maximum ultrastructural 3D resolution to visualize viruses, bacteria, macromolecular complexes, and subcellular components for the study of host-pathogen interactions. This improved ability offers significant structural information, improving our ability to relate structure to function. The 300 kV microscope with automated stage, tomography package, and energy filtering systems improve resolution in all axes and give us the ability to bridge the imaging gap between confocal microscopy and electron microscopy and to expand toward molecular imaging.​

FEI/Titan Krios 300kV TEM
Credit: NIAID

FEI/Titan Krios 300kV TEM Enables higher resolution for 2- and 3-D imaging of room-temperature or cryo specimens less than or equal to 1 micron

3D Tomography

Tomographic rendering of Chlamydia trachomatis within an inclusion vacuole of a HeLa cell.

A three-dimensional tomographic reconstruction of a tick-borne flavivirus replication complex within the endoplasmic reticulum of a persistently infected tick cell.

Ortho slices and rendering of polio virus replication complexes in a HeLa cell

Content last reviewed on August 16, 2013