Owen Schwartz, Ph.D.

Chief, Biological Imaging Section

Major Areas of Expertise

  • Confocal microscopy
  • Fluorescence microscopy
  • Video microscopy
  • Post-collection quantification and deconvolution

Program Description

The Biological Imaging Section enables intramural NIAID investigators to use state-of-the art microscopy in their research programs. We provide expertise, instruction, and instrumentation. Our staff is available to assist in experiment design and in the collection and analysis of images of living and fixed material.

Instrumentation

Our facility is equipped with specialized instrumentation dedicated to the science and art of biological imaging, which entails answering biological questions by following events as they take place inside an organism.

General Microscopy

  • Leica Episcope (#1)
  • Leica Epi (#2)
  • Leica Epi (PCO)
  • Leica AF 6000 LX TIRF
  • Leica AMS-LMD (Laser Microdissection)
  • Leica M205 FA (motorized stereo microscope)
  • Leica Epi (TW3)

Confocal Microscopy

  • Leica SP5 X-WLL
  • Leica TCS STED/FLIM (MP)
  • Leica SP8 -690
  • Leica SP8 690/730
  • Leica SP5 (BSL-3, MP)
  • Leica SP8 dual MP (BSL-2, MP)
  • Leica SP8 DIVE (BSL-2, MP)
  • Leica SP8 FLIM (TW3)

New Super Resolution Microscopy: Our facility has now entered the world of super-resolution with the installation of our new SP5-STED microscope. Until now the smallest objects one could resolve by conventional optical microscopy were 200nm across. Stimulated Emission Depletion (STED) microscopy allows the resolution limit to be lowered to approximately 65nm while still working with fluorescent probes.

  • Leica SP5
  • Leica SP2
  • Leica SP5 (BSL-3, MP)
  • Leica SP2 (BSL-2, MP)
  • Leica SP5 (BSL-2, MP)
  • Zeiss LSM 5 Live (BSL-2)

Poster Printing

  • HP DesignJet L260

Sample Preparation Equipment

  • Stereomicroscope M8
  • Leica CM1900 Cryostat (BSL-2)
  • Leica Vibratome VT1000 S (BSL-2)
  • Leica Vibratome VT1200S
  • Leica MZ95 Stereomicroscope
  • Image Processing and Analysis

Image Processing and Analysis

  • Post-collection image processing and analysis

Image Capture

Consultation

Instrument Specifications

Long Time-Lapse Imaging Chamber

NIAID Imaris Licenses

Research Group

Owen M. Schwartz, Ph.D.
Juraj Kabat, Ph.D. 
Sundar Ganesan, Ph.D.
Margery Smelkinson, Ph.D.

Content last reviewed on June 25, 2019