Owen Schwartz, Ph.D.

Chief, Biological Imaging Section

Major Areas of Expertise

  • Confocal microscopy
  • Fluorescence microscopy
  • Intravital imaging
  • Fluorescence lifetime imaging
  • Post-collection quantification and deconvolution
     

Program Description

The Biological Imaging Section enables intramural NIAID investigators to use state-of-the art microscopy in their research programs. We provide expertise, instruction, and instrumentation. Our staff is available to assist in experiment design and in the collection and analysis of images of living and fixed material.

Instrumentation





Confocal microscope image of a human artery

Single optical plane image of human artery using 25x objective. MP microscopy in situ.

Credit
NIAID

Single optical plane image of human artery using 25x objective. MP microscopy in situ.

Credit:
NIAID

Our facility is equipped with specialized instrumentation dedicated to the science and art of biological imaging, which entails answering biological questions by following events as they take place inside an organism.

General Microscopy

  • Leica Episcope (#1)
  • Leica Epi (#2)
  • Leica Epi (PCO)
  • Leica DMI8 TIRF
  • Leica AMS-LMD (Laser Microdissection)
  • Leica M205 FA (motorized stereo microscope)
  • Leica Epi (TW3)

Confocal Microscopy

  • Leica SP8 X-WLL w/FLIM
  • Leica Stellaris 8 FLIM
  • Leica SP8 -690
  • Leica SP8 690/730
  • Leica SP5 (BSL-3, MP)
  • Leica SP8 dual MP (BSL-2, MP)
  • Leica SP8 DIVE (BSL-2, MP), w/FLIM, and 4 tunable external detectors
  • Leica SP8 FLIM (TW3)

Super Resolution Microscopy: Our facility has now entered the world of super-resolution with a co-operative agreement giving access to a Leica STED 3D instrument. Until now the smallest objects one could resolve by conventional optical microscopy were 200nm across. Stimulated Emission Depletion (STED) microscopy allows the resolution limit to be lowered to approximately 65nm while still working with fluorescent probes.

Advanced Intravital Microscopy: Observation of the immune response to infectious disease is important to understanding of the underlying biological processes.  The Biological Imaging Section has 3 state-of-the-art multiphoton imaging systems for intravital imaging.    All three instruments are equipped with microscope enclosures for 37C incubation.   Two of the instruments have dual IR lasers for excitation of probes across the IR spectrum.  Additionally, the BIS is one of only a few laboratories in the world where a complete multiphoton confocal microscope is available in a biosafety level (BSL)-3 environment.

Poster Printing

  • HP DesignJet L260

Sample Preparation Equipment

  • Stereomicroscope M8
  • Leica CM1900 Cryostat (BSL-2)
  • Leica Vibratome VT1000 S (BSL-2)
  • Leica Vibratome VT1200S
  • (2) Leica MZ9.5 surgical Stereomicroscopes
  • Image Processing and Analysis

Image Processing and Analysis

  • Post-collection image processing and analysis

Image Capture

Consultation

Instrument Specifications

Long Time-Lapse Imaging Chamber

NIAID Imaris Licenses

NIAID Huygens Licenses

Research Group





Image of BIS group members

Left-to-right: Dr. Owen M Schwartz, Dr. Juraj Kabat, Dr. Anthony Fauci, Dr. Olena Kamenyeva, Dr. Sundar Ganesan, Dr. Margery Smelkinson

Credit
NIAID

Left-to-right: Dr. Owen M Schwartz, Dr. Juraj Kabat, Dr. Anthony Fauci, Dr. Olena Kamenyeva, Dr. Sundar Ganesan, Dr. Margery Smelkinson

Credit:
NIAID

 

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