Robert J. Hohman, Ph.D.

Chief, Research Technologies Branch
Chief, Protein Chemistry Section

Major Areas of Expertise

  • Analytical mass spectrometry (MS)
  • Protein identification
  • Protein separation
  • Assay development
  • Proteomics
  • Edman (N-terminal) protein sequencing
  • Peptide synthesis
  • Bioinformatics

Program Description

The Protein Chemistry Section enables intramural NIAID investigators to use state-of-the-art applications in mass spectrometry, peptide synthesis, and protein.

Instrumentation

Mass Spectrometry

  • Thermo Scientific Orbitrap Fusion Tribrid Mass Spectrometer equipped with Nano-LC Nano-Electrospray source 
  • Thermo Scientific LTQ Velos Orbitrap Mass Spectrometer equipped with Nano-LC Nano-Electrospray source
  • Bruker Microflex LRF matrix-assisted laser desorption ionization (MALDI) - time of flight (TOF) MS
  • Applied Biosystems Sciex 4000 Q Trap mass spectrometer

Peptide Synthesis

  • Applied Biosystems Model 433A Automated Peptide Synthesizer (with maximum synthetic capability of 0.25mM)
  • Protein Technologies Inc. Model “TRIBUTE-UV IR” Automated Peptide Synthesizer with UV Monitoring and IR Heat (with maximum synthetic capability of 0.20mM)
  • Bruker microflex MALDI-TOF Mass Spectrometer
  • Agilent 1200 Analytical UPLC System with Diode Array Detector and fraction collector
  • Shimadzu VP series Analytical HPLC System with Diode Array Detector
  • Agilent 1100 Preparative HPLC System with Diode Array Detector and a Single Quad Mass Spectrometer Detector
  • VirTis FREEZEMOBILE 25XL Lyophilizer

Protein Sequencing

  • Applied Biosystems 494clc Procise protein sequencer with four cartridge capability
  • Applied Biosystems 494 Procise protein sequencer with four cartridge capability

Research Group

Motoshi Suzuki, Ph.D.
Glenn Nardone, Ph.D.
Jan Lukszo, Ph.D.
L. Renee Olano, Ph.D
Ming Zhao, Ph.D.
Brian Martin, Ph.D.

Specialties

Motoshi Suzuki, Ph.D., Mass Spectrometry
L. Renee Olano, Ph.D., Bioinformatics
Jan Lukszo, Ph.D., Peptide Synthesis and Analysis 
Glenn Nardone, Ph.D., Assay Development
Ming Zhao, Ph.D., Protein Separation
Brian Martin, Ph.D, Edman Sequencing

Content last reviewed on November 6, 2017