Tom Moyer

Acting Chief, Flow Cytometry Section

Major Areas of Research

  • Flow cytometric analysis (up to 13-color sorting, up to 14-color analysis, BSL-3 sorting and analysis, multispectral imaging cytometry, and multiplex bead array assays)
  • Sorting technologies

Program Description

The Flow Cytometry Section (FCS) provides DIR with application-specific flow cytometric technologies, including instrumentation and multi-color analysis. In addition, the FCS provides training, consultation, method development, and analysis for experiments involving flow cytometry. This mission is accomplished through the efforts of staff with extensive flow cytometry experience and with the use of six cell sorters and eight analyzers. Major goals are to provide access to state-of-the-art technologies, to help design and run experiments, to facilitate data interpretation, and to provide results that are of consistent high quality.

Instrumentation

Main facility - NIH campus (Building 4)

Cell Sorters:

  • BD FACS Aria Fusion-Orange
    • 15-color capability,
    • 5 lasers: 355nm, 405nm, 445nm, 488nm, 561nm, and 640nm
    • Located in Class II Biological Safety Cabinet
  • BD FACS Aria III-Red cell sorter
    • 17-color capability
    • 4 lasers: 405nm, 488nm, 561nm, and 640nm
    • Located in Class II Biological Safety Cabinet
  • BD FACS Aria III-Blue cell sorter
    • 15-color capability
    • 4 lasers: 405nm, 488nm, 561nm, and 638nm

Analyzers:

  • BD LSR II
    • 18-color capability
    • 4 lasers: 355nm, 405nm, 488nm, and 640nm
  • BD Symphony (New in FY 16)
    • 28-color capability
    • 5 lasers: 355nm, 405nm, 488nm, 532nm, and 628nm

NIH campus (Building 50)

  • BD FACS CANTO
    • 8-color capability
    • 3 lasers: 405nm, 488nm, and 640nm
  • AMNIS ImagestreamX Mark II
    • 3 Laser, 9-color capability, dark field and brightfield
  • Millipore MagPix
    • Up to 50 analytes/sample

Twinbrook II

  • BD LSRII
    • 18-color capability
    • 4 lasers: 405nm, 488nm, 532nm and 640nm

NIH campus (Building 33)

Cell Sorters – BSL2:

  • BSL2 FACS Aria II-Emerald Special Order cell sorter
    • 16-color capability
    • 4 lasers: 405nm, 488n, 532nm, and 640nm
  • BSL2 FACS Aria II-Cyan Special Order cell sorter
    • 13-color capability
    • 4 lasers: 405nm, 488nm, 561nm, and 640nm

Cell sorters – BSL3:

With the increase in demand for high-speed cell sorting of viable infectious samples in the DIR, it is important for NIAID to have infectious cell sorting capabilities in BSL-3 laboratories. There are only a handful of BSL3 cell sorters worldwide, so the DIR is fortunate to have the instrumentation and expertise on campus.

  • BSL3 FACS Aria II-Silver cell sorter (both select and non-select agent BSL3 pathogens)

    • 11-color capability
    • 3 lasers: 405nm, 488nm, and 640nm

Analyzers:

  • BD LSR Fortessa (Symphony electronics upgrade; New in FY 16))
    • 18-color capability
    • 4 lasers: 405nm, 488nm, 532nm, and 640nm
  • BSL3 BD LSR II with HTS
    • 12-color capability
    • 3 lasers: 405nm, 488nm, and 640nm

Research Group

  • Dareskedar Admassu, M.S.
  • Calvin Eigsti, B.A.
  • Teresa Hawley, B.S.
  • Bishop Hague, Ph.D.
  • Carol Henry, B.S. 
  • Larry M. Lantz, Ph.D.
  • David Stephany, B.S.

Specialties

  • Custom Antibodies, Larry M. Lantz, Ph.D.
  • We also provide a cost- and time-efficient method of obtaining fluorescent-labeled antibodies. Resources are available to all intramural NIAID investigators and include
    • Hybridoma expansion
    • Antibody purification from ascites, antisera, and hybridoma supernatant
    • Coupling of purified antibodies and proteins to various flurochromes
Content last reviewed on November 8, 2017