Pathology Core at the Integrated Research Facility

Major Areas of Research

  • Gross pathology, including high-containment/biosafety level (BSL)-4 necropsy
  • Histopathology
  • Electron microscopy

Program Description

The Pathology Core laboratories include two state-of-the-art BSL-4 necropsy suites and full-service histopathology and electron microscopy laboratories. We are equipped to perform complete high-containment/BSL-4 postmortem examinations on a variety of species, including rodents and nonhuman primates. Our fully digitized pathology core facility provides real-time gross pathology images from the postmortem table and gross tissue trimming stations using the MacroVIEW D imaging system.

Our fully automated histopathology laboratory provides standard hematoxylin and eosin-stained tissue sections, myriad special histochemical stains, immunohistochemical staining using chromogenic and fluorescent dyes, and in situ hybridization. All of our stained tissue sections are digitally scanned and archived as whole slide images using the Leica SCN400/SL801 scanner. We provide quantitative manual and/or colormetric digital image analysis of immunohistochemically stained slides using Image Pro Analyzer 7.0, SlidePath Tissue IA 3.1, and the Nuance FX multispectral imaging system. We facilitate cell type-specific molecular quantitative analysis of nucleic acids and proteins using the Leica LMD 6500 laser capture microdissection system.

We are also a full service electron microscopy laboratory, providing a variety of embedding resins and processing techniques for both transmission and scanning electron microscopy, including immunogold labeling of individual epitopes in both in vitro cell and ex vivo tissue sections using the Leica EM IGL labeling system and virus particle quantification using the Beckman-Coulter Airfuge. Our FEI Tecnai G2 Spirit Twin Transmission Electron Microscope (TEM) provides both 2D and 3D tomography images. We can produce high-resolution, low-artifact cryo-TEM sections using the FEI Vitrobot. Our FEI Quanta 3D FEG Dual Beam Scanning Electron Microscope also provides both 2D and 3D images using the Resolve RT 3D software. In addition, we provide high-resolution, publication-quality brightfield or darkfield photomicrographs of up to 12-megapixel resolution using Leica DFC500 digital cameras through planar Leica DM3000 objectives.

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