Put your research plans in scientific context for your reviewers and convince them that you know your field by citing wisely in your application, including a Bibliography, and including appropriate Appendix materials.
Include a biographical sketch for every person who plays a substantive role in your research. That includes consultants and technical staff, even if they are not paid a salary from the grant.
Does your application need a cover letter? In some cases, it does, in others, it is optional. Note that only the scientific review officer sees your cover letter, not the reviewers or program officers.
Your application may need to include a plan for sharing model organisms, final research data, or data or other information related to genome-wide association studies.
Use the optional PHS Assignment Request Form to list expertise needed to review your application, exclude reviewers, and request an institute assignment.