Biocontainment Laboratory: Colorado State University Infectious Disease Research Center

The Colorado State University Regional Biocontainment Laboratory is one of the NIAID-supported Regional Biocontainment Laboratories. The Center provides a safe, secure, state of art facility for university investigators, government scientists and industry representatives to collaboratively research bacteria and viruses that cause human and animal diseases

Main Areas of Focus

  • To research the basic biology, biochemistry, molecular biology and epidemiology of bacteria and viruses that cause human and animal diseases.
  • To provide outstanding service to research and small business in order to support and their research goals.

Who Can Use This Resource

  • Investigators in academia, not-for-profit organizations, industry, and government studying biodefense and emerging infectious diseases may request the use of biocontainment laboratories.

How To Get Started

  • Please contact the IRDC directly.

Support Services

Laboratory and Analytical Support

  • Immunology services: Flow Cytometry, Cytokine Bead array
  • Pathology services: Available via Veterinary Diagnostic Lab on campus
  • Microbiology services: Available via Veterinary Diagnostic Lab on campus 

Ability To Accommodate cGLP or cGMP Studies?

  • Both cGLP and cGMP are available

Tools

Animal Models, Including Species, Disease, and Delivery Method

A=aerosol, IN=intranasal, S=subcutaneous, O=oral, IP=intraperitoneal, T=transthorasic, IC=intracerebral, ID=intradermal, IM= intramuscular, IT= intratracheal, IV=intravenous, M=mosquito, IN=intranasal, INH=intrahepatic, INR=intratracheal

  • Mice: F. tularensis (aerosol, intranasal, intradermal), Y. pestis (intranasal, sc), VEE virus (sc, aerosol), WEE virus (sc, aerosol), West Nile virus (sc, ip), C. immitis (intranasal), highly pathogenic avian influenza (intranasal)
  • Goats: Burkholderia pseudomallei (intranasal,sc), B.mallei (intranasal), Brucella abortus (ip), B. anthracis (in, sc), Mycobacterium tuberculosis (inhalation, intranasal)
  • Hamsters: Japanese encephalitis virus (sc), West Nile virus (sc, ip)
  • Rabbits: M. tuberculosis (inhalation)
  • Ferrets: seasonal (H1/N3); highly pathogenic (H5/N1) and swine origin (H1N1) influenza viruses (intranasal)
  • Bats: (various viruses)

Pathogens

  • Burkholderia mallei
  • Burkholderia pseudomallei
  • Yersinia pestis
  • Francisella tularensis
  • VEE virus
  • EEE virus
  • WEE virus
  • Rift Valley fever virus
  • Japanese encephalitis virus
  • Coccidioides immitis
  • Yellow fever virus
  • West Nile virus
  • Highly pathogenic avian influenza virus
  • Seasonal human influenza A viruses
  • Rabies virus
  • Mycobacterium tuberculosis
  • Mycobacterium bovis

Imaging Capabilities

  • Xenogen IVIS 100 Series machine with a 25 mm square cryogenically cooled CCD camera, contained within BSL-3 space

Major Equipment Available

The Proteomics/Metabolomics Facility Laboratory has the following:

  • Nanodrop spectrophotometer
  • GenePix 4000B microarray scanner
  • Agilent Bioanalyzer 2100
  • Agilent Q-TOF mass spectrometer
  • Agilent 1200 series liquid chromatograph system
  • BioTek Synergy 2 multi-detection microplate reader
  • ChemiDoc XRS multicolor fluorescent imaging system
  • Eppendorf EP Motion 5075 VAC Liquid handling robotics system
  • Qpix Robot
  • BioRad ChipWriter Pro microarray printer

Shared Resources

The facility is part of a 120,000 square foot Infectious Disease Research Center. Within the IDRC are multiple ABSL-3 animal handling rooms that comprises the Animal Models core with the capability of dealing with a multiplicity of animals, agents and delivery modalities, and a Next Generation Sequencing Core with an Illumina NextSeq, Illumina MiSeq, Ion Proton and Ion Torrent instruments.

An 8,200 square foot cGMP manufacturing building is on site and validated, and there is an imaging facility designed for BSL 3 housing of MRI and SPEC-CT machines contiguous with the BSL 3 space. ​​​​​​

Content last reviewed on November 17, 2017