Correlative Light and Electron Microscopy (CLEM)

CLEM overcomes the resolution limitations of light microscopy by enabling electron microscopic analysis of an identified region for high-resolution analysis. This technique involves processing specimens for light microscopy and imaging by fluorescence followed by preparation for and imaging by electron microscopy. As a result, the quenching of fluorophores during electron microscopy processing is no longer a concern.

CLEM can be used in combination with both scanning and transmission electron microscopy techniques.

CLEM SEM

Image of HeLa cells

CLEM TEM

Image of HeLa cells

Content last reviewed on August 16, 2013