Correlative Light and Electron Microscopy (CLEM)

CLEM overcomes the resolution limitations of light microscopy by enabling electron microscopic analysis of an identified region for high-resolution analysis. This technique involves processing specimens for light microscopy and imaging by fluorescence followed by preparation for and imaging by electron microscopy. As a result, the quenching of fluorophores during electron microscopy processing is no longer a concern.

CLEM can be used in combination with both scanning and transmission electron microscopy techniques.

CLEM SEM

Image of HeLa cells
Credit: NIAID

HeLa cells were grown on formvar-coated Ni finder grids and infected with mCherry-transformed Salmonella bacteria (pseudo colored green).

CLEM TEM

Image of HeLa cells
Credit: NIAID

HeLa cells infected with Chlamydia trachomatis grown on photo-etched glass coverslips and labeled with fluorescent tagged anti-elementary body antibody. The TEM images reveal higher resolution information of the cells imaged by light microscopy.

Content last reviewed on August 16, 2013