The Duke University School of Medicine, Duke Human Vaccine Institute (DHVI) Regional Biocontainment Lab (RBL) is one of the NIAID-supported Biocontainment Laboratories.
The RBL supports basic research to develop drugs, diagnostics, and vaccines for emerging and reemerging infections and biodefense. The RBL has a comprehensive safety and operations program to provide state-of-the-art biocontainment facilities for BSL2, BSL3, and Select Agent research
Main Areas of Focus
- To support the advancement of biodefense and infectious disease research
- To support research programs in the areas of
- Safety and biopreparedness
- Vaccine/therapeutic development
- Host response/immune monitoring
- Proficiency testing/ quality assurance
Who Can Use This Resource
- Investigators in academia, not-for-profit organizations, industry, and government studying biodefense and emerging infectious diseases may request the use of biocontainment laboratories.
How To Get Started
- Please contact the RBL directly for further information.
Laboratory and Analytical Support
All of the following are available at enhanced BSL3/ABSL3:
- Immunology: In vitro functional assays, ELISA, live cytometry and cell sorting, multiplex biomarker analysis (Luminex), and real-time PCR arrays
- Virology: Stock preparation and assay development (focus-forming units, TCID50, plaque-forming units); influenza hemagglutination inhibition and micro-neutralization assays
- Bacteriology: Inoculum preparation and validation, colony-forming units
- Pathology services: Sample collection and necropsy for tissue collection/fixation, access to Duke Department of Pathology core lab for embedding/cutting
Ability To Accommodate cGLP or cGMP Studies?
- Current GLP: HIV propagation and assays with quality control for proficiency panels; Luminex biomarker assays and Luminex proficiency testing (accredited to the ISO/IEC 17043:2010 standards by the American Association for Laboratory Accreditation [A2LA])
- Current cGMP: None
Animal Capabilities, Including Species and Containment Level(s)
Enhanced ABSL3 housing available for mice, rats, guinea pigs, and rabbits.
Animal Models, Including Species, Disease, and Delivery Method
All current models are in mice:
- Yersinia pestis CO92, BSL3 , aerosol, anterior cervical, footpad
- Mycobacterium tuberculosis multiple strains (sensitive, MDR, XDR), BSL3, aerosol
- West Nile virus IC7 & NY99, BSL3, footpad, intraperitoneal
- Francisella tularensis SchuS4, BSL3, aerosol, intradermal
- Vaccinia virus WR and Cowpox virus Brighton Red, BSL2, intranasal
- Influenza virus PR8, BSL2, intranasal
- Cryptococcus gattii and C. neoformans multiple, BSL2, aerosol
- Current Select Agent approval:
- Bacillus anthracis Ames
- Brucella abortis
- Brucella suis
- Brucella melitensis
- Burkholderia pseudomallei
- Francisella tularensis
- Highly pathogenic avian influenza
- Monkeypox virus
- Yersinia pestis
- Other agents handled in the facility:
- Mycobacterium tuberculosis (sensitive, MDR, XDR)
- Staphylococcus aureus
- West Nile virus
- Vaccinia virus,
- Cowpox virus
- Rabbitpox virus
- Human influenza viruses (H1N1, H3N2, H7N9)
- Low pathogenic avian influenza virus
- Human rotavirus
- Vesicular stomatitis virus
- Yellow fever virus
- Cryptococcus gattii
- C. neoformans
- N. gonorrhoeae
- C. trachomatis
Spectrum In Vivo Imaging System (IVIS, Perkin Elmer) with both luminescence and fluorescence detection, validated for mice at enhanced ABSL3 (Appl Biosaf. 2010;15(2):62-66; PMCID: 3515055)
Major Equipment Available
All of the following specialized resources and major equipment are installed, validated, and available at enhanced BSL3/ABSL3:
- BD FACSAria IIu cell sorter; Luminex bead array reader; real-time Thermal Cycler, ELISA plate reader and washer.
- Two whole-body aerosol exposure systems, each with capacity for simultaneous exposure of 144 mice, 18 guinea pigs, 6 rabbits, or similar numbers of other small animals and one nose-only exposure system for exposure of 36 mice.
- Aerosol Management Platform (AeroMP, Biaera) to control whole-body and nose-only exposures (controls and records flow, pressure, humidity, and temperature).
- Aerodynamic Particle Sizer (APS, TSI) for real-time monitoring of particle number and size distribution (count or mass) in infectious aerosols.
- Biosamplers and impingers to determine concentration of viable bacteria or yeast in infectious aerosols.
Shared Resources/Core Labs
The faculty and senior scientific staff of the Duke Human Vaccine Institute have assembled state-of-the-art technologies into Shared Resources (i. e. Core Labs) to accelerate research. These comprehensive Shared Resources are available to the entire Duke community and their collaborators. DHVI cores are supported with institutional funds and a variety of shared instrumentation grants, center grants, and program awards.
The RBL at Duke functions administratively as one of the eight DHVI Shared Resources and this membership allows RBL users access to a wide-range of cutting edge technologies and facilities to enhance their research.
- Regional Biocontainment Laboratory: Home to a portfolio of collaborative research units focusing on Bacteriology, Virology, and Immunology. Fully-commissioned for BSL3/Select Agent work in late 2007 and has supported over 60 publications.
- Research Flow Cytometry & Cell Sorting: Analytical/sorting on six BD instruments with up to 23 simultaneous parameters.
- Biomolecular Interaction Analysis: State-of-the-art Surface Plasmon Resonance (SPR) BIAcore instruments for monitoring real-time interaction analyses and measurement of binding affinity, kinetics, and epitope mapping.
- Immunology Virology Quality Assessment Center Laboratory: Delivery of CAP- and CLiA-certified immunologic and viral load assays (i. e. immunophenotyping; SIV viral load, HIV serology, HIV and HCV viral load; HIV acute infection disease staging, and large-scale cell separation.
- Viral Genetic Analysis: Large-scale DNA sequencing, Next-Gen sequencing, and sequence data analysis, to serve the viral genetic analysis needs of researchers.
- X-ray Crystallography: Automated crystallization systems and high resolution X-ray diffraction image collection to solve macromolecular crystal structures (crystallization trials, data collection, phasing, refinement, and analysis).
- Protein Production Facility: Quality and standard recombinant protein production under “GxP best practices” or in vitro use.
- Accessioning Unit and Clinical Trials Support: Centralized support for preparation of human clinical studies, collection of human specimens, and ongoing compliance oversight. Processing and continuous auditable storage/monitoring of frozen samples derived from studies/trials.