Major Areas of Research
- 3D Tomography and High-Resolution TEM
- Confocal Microscopy
- Correlative Light and Electron Microscopy (CLEM)
- Cryo-Electron Microscopy
- Immuno-Electron Microscopy
- Scanning Electron Microscopy (SEM)
- Transmission Electron Microscopy (TEM)
The Electron Microscopy (EM) Unit provides state-of-the-art technologies to support the ultrastructural imaging needs of NIAID scientists both in the Bethesda, Maryland, area and at the Rocky Mountain Laboratories in Hamilton, Montana.
The facility provides sample preparation and analysis ranging from basic to high resolution structural studies to immuno-localization of selected antigens for a wide array of specimens. A variety of methods, protocols, and equipment are employed to accommodate different preparative and imaging needs.
Chiramel AI, Meyerson NR, McNally KL, Broeckel RM, Montoya VR, Méndez-Solís O, Robertson SJ, Sturdevant GL, Lubick KJ, Nair V, Youseff BH, Ireland RM, Bosio CM, Kim K, Luban J, Hirsch VM, Taylor RT, Bouamr F, Sawyer SL, Best SM. TRIM5α Restricts Flavivirus Replication by Targeting the Viral Protease for Proteasomal Degradation. Cell Rep. 2019 Jun 11;27(11):3269-3283.e6. doi: 10.1016/j.celrep.2019.05.040
Chlanda P, Mekhedov E, Waters H, Schwartz CL, Fischer ER, Ryham RJ, Cohen FS, Blank PS, Zimmerberg J. The hemifusion structure induced by influenza virus haemagglutinin is determined by physical properties of the target membranes.Nat Microbiol. 2016 Apr 18;1(6):16050. doi: 10.1038/nmicrobiol.2016.50.
Chen Z, Fischer ER, Kouiavskaia D, Hansen BT, Ludtke SJ, Bidzhieva B, Makiya M, Agulto L, Purcell RH, Chumakov K. Cross-neutralizing human anti-poliovirus antibodies bind the recognition site for cellular receptor. Proc Natl Acad Sci U S A. 2013 Dec 10;110(50):20242-7. doi: 10.1073/pnas.1320041110. Epub 2013 Nov 25. PMID:24277851
Fischer ER, Hansen BT, Nair V, Hoyt FH, Dorward DW. Scanning electron microscopy. Curr Protoc Microbiol. 2012 May;Chapter 2:Unit 2B.2.
Belov GA, Nair V, Hansen BT, Hoyt FH, Fischer ER, Ehrenfeld E. Complex dynamic development of poliovirus membranous replication complexes. J Virol. 2012 Jan;86(1):302-12.
The RML Microscopy Unit has state-of-the-art microscopy equipment, including two scanning electron microscopes, a high-pressure freezer, high-resolution transmission electron microscopes with 3D tomography capabilities, and more. Instruments include from Thermo Fisher a 300 kV Titan Krios with a Falcon 3 detector, the 120 kV Tecnai and the Helios G4 focused-ion beam SEM. Hitachi instruments include the H-7800 120 kV TEM and the SU-8000 SEM. Light microscopy facility includes a Zeiss 880 LSM with airyscan.
Single-particle reconstruction of Norovirus. Data collected on the Titan Krios using the direct electron detector, Falcon II, allowed reconstruction of Norovirus at 5.8 angstrom resolution from 1696 imaged particles. NIAID P.I. Dr. Kim Green
Mycobacterium tuberculosis (Mtb) in lung tissue within granuloma excised from a rabbit. Dual-beam data sets collected on a FEI Helios dual beam microscope allowed reconstruction of whole Mtb in the context of an in vivo infection revealing its encapsulation in a lipid rich environment. This is an important consideration when developing drug delivery schemes. NIAID P.I. Dr. Clifton Barry III.