Major Areas of Research
The Electron Microscopy (EM) Unit is dedicated to providing state-of-the-art technologies to support the ultrastructural imaging needs of NIAID scientists both in the Bethesda, Maryland, area and at the Rocky Mountain Laboratories in Hamilton, Montana.
The facility provides sample preparation and analysis ranging from basic to high resolution structural studies to immuno-localization of selected antigens for a wide array of specimens. A variety of methods, protocols, and equipment are employed to accommodate different preparative and imaging needs. Real-time viewing of samples by video image-sharing is available to NIAID users, enabling scientists at distant locations the ability to view samples simultaneously with microscopy staff.
Chen Z, Fischer ER, Kouiavskaia D, Hansen BT, Ludtke SJ, Bidzhieva B, Makiya M, Agulto L, Purcell RH, Chumakov K. Cross-neutralizing human anti-poliovirus antibodies bind the recognition site for cellular receptor. Proc Natl Acad Sci U S A. 2013 Dec 10;110(50):20242-7. doi: 10.1073/pnas.1320041110. Epub 2013 Nov 25. PMID:24277851
Ngamskulrungroj P, Chang Y, Hansen B, Bugge C, Fischer E, Kwon-Chung KJ. Characterization of the chromosome 4 genes that affect fluconazole-induced disomy formation in Cryptococcus neoformans. PLoS One. 2012;7(3):e33022.
Offerdahl DK, Dorward DW, Hansen BT, Bloom ME. A three-dimensional comparison of tick-borne flavivirus infection in mammalian and tick cell lines. PLoS One. 2012;7(10):e47912
Fischer ER, Hansen BT, Nair V, Hoyt FH, Dorward DW. Scanning electron microscopy. Curr Protoc Microbiol. 2012 May;Chapter 2:Unit 2B.2.
Belov GA, Nair V, Hansen BT, Hoyt FH, Fischer ER, Ehrenfeld E. Complex dynamic development of poliovirus membranous replication complexes. J Virol. 2012 Jan;86(1):302-12.
Cocchiaro JL, Kumar Y, Fischer ER, Hackstadt T, Valdivia RH. Cytoplasmic lipid droplets are translocated into the lumen of theChlamydia trachomatis parasitophorous vacuole. Proc Natl Acad Sci U S A. 2008 Jul8;105(27):9379-84.
The RML Microscopy Unit has state-of-the-art microscopy equipment, including two scanning electron microscopes, a high-pressure freezer, high-resolution transmission electron microscopes with 3D tomography capabilities, and more.