SOPs for In Vitro Culture System for Cryptosporidium

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NIAID-funded researchers at ATCC and Tufts University have developed SOPs for creating continuous in vitro culture systems for the intestinal parasite Cryptosporidium. Drug development has been hampered by limitations of methods to propagate the organism in vitro. The following SOPs describe procedures to establish and maintain Cryptosporidium hominis and Cryptosporidium parvum cultures.

Main Areas of Focus

  • Standard operating procedures (SOPs) for Cryptosporidium cultures

Who Can Use This Resource

  • Available to the scientific community

SOP Documents

  • SOP: Development of Continuous in vitro Culture System for Cryptosporidium – Tufts University
    This SOP provides information on the continuous in vitro culture of C. hominis in COLO-680N cells using hollow fiber technology system, and the continuous in vitro culture of C. parvum in HCT-8 cells using hollow fiber technology System
  • SOP: Development of Continuous in vitro Culture System for Cryptosporidium – ATCC
    This collection of SOPs provides information on processes including: the isolation of primary human intestinal epithelial cells; isolation of primary intestinal epithelium from laboratory mice; growing on polycarbonate membrane supports; infected epithelial cells growing on polycarbonate membrane supports;  human intestinal organoid isolation; mouse intestinal organoid culture;  inoculation of mouse intestinal organoids with C. parvum; and transmission electron microscopy for C. parvum-infected intestinal epithelial cells growing on polycarbonate membrane supports   
Content last reviewed on November 5, 2018