The University of Missouri Laboratory for Infectious Disease Research (LIDR) is one of the NIAID-supported Biocontainment Laboratories. LIDR fosters research by University of Missouri faculty and collaborators for novel vaccines, diagnostics, and therapeutics to combat emerging infectious diseases and biological weapons. Dedicated aerobiology, arthropod, and immunology/imaging suites, along with an ABLS-3 vivarium, procedure rooms, and auxiliary labs, provide researchers the necessary state-of-the-art equipment and secure space to conduct cutting-edge translational research. The facility, constructed to the highest state and federal safety standards, and central location make LIDR a crucial resource in our nation’s effort to protect public health.
Main Areas of Focus
- To advance innovative research for preventing and treating diseases caused by deadly pathogens
- To further collaborative research with other academic or industry partners
- To increase research capacity and educational programs for the safe handling of high-consequence biological agents
- To prepare the next generation of infectious disease scientists
- To serve as a regional and national resource in the event of a biological agent release or infectious disease outbreak
Who Can Use This Resource
- Investigators in academia, not-for-profit organizations, industry, and government studying biodefense and emerging infectious diseases may request the use of biocontainment laboratories.
Laboratory and Analytical Support
Customized aerosol delivery services for BSL-2, BSL-3, and non-infectious agents using a nose-only inhalation exposure system inside a Class III biological safety cabinet connected directly from the aerobiology suite to the vivarium via an interlocked pass-through chamber:
- Aerosol Characterization including Dose Targeting and Determination
- Inoculation Preparation and Challenge Experiments
- Nose-only Inhalation Exposure
- Pathogen and Small Animal Model Development
- Vaccine and Therapeutic Efficacy Validation
Immune response evaluation services for fixed/live BSL-1, BSL-2, and BSL-3 samples supported by a flow cytometer, Luminex Magpix, automated microscope complete with environmental control, and fluorescent microscopes:
- Consultation and Experiment Design Assistance
- Fluorescence, Brightfield/Color Brightfield, and Phase Contrast Microscopy
- Four-way Bulk or Single-cell Sorting onto Slides or Multi-well Plates
- Long-term, Live-cell Imaging
- Quantitative and Time-lapse Cellular Response Analysis
- Vaccine or Infection Immune Response Analysis
- Multiplex immunoassays for over 100 Cytokine, Chemokine, and Growth Factor Targets
- Other- ELISA, ELISPOT, Post-mortem Cytokine Analysis
- Microbiology- Antimicrobial/Antiviral Susceptibility Testing; Bacterial/Viral Preparation; DNA Manipulation; Inoculum Preparation and Validation; qPCR; Tissue/Cell Culture
- Pathology- Necropsy; Gross Pathology; Post-mortem Tissue Burden Pathogen Analysis; Histopathology; Immunohistochemistry; Liver, Renal, and Serum Clinical Chemistries
- Vector Biology- Arthropod Containment Level 3 (ACL-3)for Vector Infection and Maintenance; Tissue Dissection; Virus Detection
- Vivarium- Animal Evaluations and Health Monitoring; Breeding; Housing; Procedure Assistance; Safety and Operations Training
Ability To Accommodate cGLP or cGMP Studies?
Animal Models, Including Species, Disease, and Delivery Methods
Current Animal Models
Mouse: B. anthracis, Brucella spp., C. burnetii, F. tularensis, O. tsutsugamushi, S. pyogenes, Y. pestis, Influenza A virus (Delivery methods: aerosol, intradermal, intranasal, intraperitoneal, oral, subcutaneous, arthropod-borne
Rat: F. tularensis, Y. pestis (Delivery methods: aerosol, intradermal, intranasal, subcutaneous)
Hamster: Zika virus (Delivery methods: subcutaneous, arthropod-borne)
Guinea Pig: B anthracis, C. burnetii (Delivery methods: aerosol, intraperitoneal, subcutaneous)
Vector Transmission Models
Mosquito: Chikungunya virus, Dengue virus, Sindbis virus, Zika virus (Delivery methods: intrathoracic, oral)
Flea: Y. pestis (Delivery method: arthropod-borne)
Tick: Heartland virus, Ehrlichias spp. (Delivery method: microinjection)
Current Select Agent Approval:
- Bacillus anthracis
- Botulinum neurotoxin-producing species of Clostridium
- Brucella abortus
- Brucella melitensis
- Brucella suis
- Burkholderia mallei
- Burkholderia pseudomallei
- Coxiella burnetii
- Francisella tularensis
- T-2 toxin
- Yersinia pestis
- Chikungunya virus
- Coccidioides immitis
- Dengue virus
- Heartland virus
- Influenza A virus
- Mayaro virus
- Mycobacterium tuberculosis
- Orientia tsutsugamushi
- Sindbis virus
- Streptococcus pyogenes
- Zika virus
- BioTek® LionheartTM FX
- Fluorescent Microscopes:
- Nikon Eclipse 50i
- Olympus BX-41
Major Equipment Available
- CH Technologies Nose-only Inhalation Exposure System (NOIES)- provides accurate and reproducible generation and delivery of aerosols. Particle size is monitored using a Palas Welas® white light aerosol spectrometer helping to target aerosols to specific regions of the respiratory tract.
- Aerosol concentration and presented dose determined by direct impingement or cascade impaction followed by growth or qPCR quantification.
- GermfreeTM Class III Biological Safety Cabinet- a custom-designed and –built, glovebox that houses the NOIES and is connected directly to the vivarium via an interlocked pass-through.
- Beckman Coulter® MoFloTM XDP- a high-speed, 3-laser/8-color cell sorter with 488nm, 561nm, and 635nm fixed-wavelength lasers; 4-way bulk sorting as well as single-cell sorting into 96-well plates or onto slides; analysis speeds up to 100,000 events per second; sorting speeds up to 70,000 events per second; and an open platform for customization of fluorochrome combinations.
- BioTek® LionheartTM FX- an automated, live-cell imager that offers cytometry and imaging of fixed or live BSL-1, BSL-2, and BSL-3 samples; fluorescence imaging (10 colors, 4 simultaneously), brightfield, color brightfield, and phase contrast; an environmental control chamber for long-term live-cell imaging; analysis in a range of tissue culture flasks, multi-well plates, and slides; and powerful Gen5 3.0 software for image capture, annotation, analysis, and movie making.
- Luminex® MAGPIX®- A compact, fluorescent-based detection system capable of processing up to 50 analytes per well with configurable simplex and multiplex immunoassay panels covering more than 100 cytokine, chemokine, growth factor, and other targets from human, mouse, rat, and other species.
- Nikon Eclipse 50i and Olympus BX-41- fluorescent microscopes with 10X, 40X, and 60X Plan Fluor objectives; filter cubes for DAPI, FITC, Cy5, and TexasRed; Q-Imaging Systems imaging software; and Q-Imaging Systems QiCam Monochrome camera for high resolution fluorescence image capture.
- Vivarium- Procedure suites with Class II biological safety cabinets; isolator caging for mice, rats, guinea pigs, rabbits, and ferrets; dedicated goat housing and procedure suite.
- Vector Suite- Arthropod Containment Level 3 for vector-borne disease research and housing.
Animal Modeling Core: provides services associated with the generation and characterization of animal models including expert advice on the design and approach for generating animal models as well as traditional techniques (e.g. embryonic stem cell modification) and cutting-edge genetic modification tools (e.g. CRISPR/Cas9 system) to generate models.
Cell and Immunobiology Core: provides services for non-infectious and fixed samples including custom monoclonal antibody production, normal and tumor cell preparation, cell acquisition and/or propagation, real-time metabolic analysis, flow cytometric services, and tissue culture reagents.
Dalton Cardiovascular Research Center: a facility dedicated to multidisciplinary collaborations among investigators from medicine, engineering, veterinary medicine, and other departments to better understand the causes of many diseases, leading to prevention and treatment.
DNA Core: provides services in DNA sequencing, DNA fragment analysis, genotyping, genomic variation analysis, and gene expression, as well as maintains the Enzyme Freezer Program for University of Missouri-Columbia.
Electron Microscopy Core: provides consultation, training, and services for scanning and transmission electron microscopy, immunoelectron microscopy, x-ray microanalysis, digital imaging and analysis, electron beam lithography, and materials and biological specimen preparation.
Gehrke Proteomics Center: provides advanced technologies in protein sequencing, custom peptide synthesizing, HPLC and capillary electrophoretic analyses, and mass spectrometry identification.
Informatics Research Core: offers a broad range of expertise and capabilities to facilitate the use of high-performance computing hardware to analyze research data.
MU Institute for Clinical and Translational Science: serves as the focal point to connect interdisciplinary researchers with research tools and provide mentoring and education opportunities to the next generation of researchers.
International Institute of Nano and Molecular Medicine: a research center dedicated to the creation of novel materials, devices, and agents through the discovery and application of fundamental and translational medical science based upon previously unexplored chemistry combined with nanotechnology and the biosciences.
Metabolomics Center: provides targeted and non-targeted small molecule/metabolite profiling and data processing, as well as user training on sample preparation, data processing, and analysis.
Metagenomics Center: provides services associated with the characterization of complex microbial communities including DNA extraction, next-generation sequencing, and study design consultation, as well as re-derivation of genetically manipulated rodents to generate animals harboring a desired complex microbiota.
Molecular Cytology Core: offers services for immunocytochemistry, laser capture microdissection, light microscopy, in situ hybridization techniques, and general scientific image analysis and processing.
Molecular Interactions Core: provides instrumentation and assistance for crystallography, dynamic light scattering, nuclear magnetic resonance, peptide synthesis, and custom liquid chromatography–mass spectrometry.
MU Mutant Mouse Resource and Research Center (one of four centers comprising the MMRRC): supplies mouse models, embryonic stem cells, related reagents, and protocols, as well as unique repository services including importing, storing, and distributing a vast number of mutant mouse strains.
MU National Swine Resource and Research Center: ensures access to critically needed swine models and serves as a central resource for reagents, creation of new genetically modified swine, and information and training related to use of swine models in biomedical research.
MU Rat Resource and Research Center: supplies rat models, embryonic stem cells, related reagents, protocols and training, and other specialized services including importing, storing, and distributing rat strains and facilitating strain acquisition from international repositories.
Nuclear Magnetic Resonance Core: assists researchers in using NMR for structural elucidation of molecules and the study of chemical and biological reactions, as well as experiment design and spectral analysis.
Research Reactor Center: a 10-megawatt reactor (the largest research reactor in the U.S.) that contributes to research on boron neutron capture therapy, neutron scattering and neutron interferometry, radioisotopes for imaging and treatment of cancer, neutron transmutation doping of semiconductor materials, epidemiology, and various other fields of medicine.
Veterinary Medical Diagnostic Laboratory: an American Association of Veterinary Laboratory Diagnosticians accredited, full-service laboratory that provides diagnostic services including anatomic and clinical pathology, bacteriology and mycology, serology, toxicology, and molecular diagnostics.