Biocontainment Laboratory: University of Missouri Regional Laboratory for Infectious Disease Research (LIDR)

The University of Missouri Laboratory for Infectious Disease Research (LIDR) is one of the NIAID-supported Biocontainment Laboratories. LIDR is a critical resource for University of Missouri faculty and collaborating scientists who perform research on infectious diseases and is part of our nation’s effort to protect public health. Built to the highest state and federal safety standards, this building aids researchers in the discovery and development of new ways to fight bacterial and viral infections.

Main Areas of Focus

  • To support research on infectious diseases.
  • To foster research in the development of novel approaches to prevent and treat diseases caused by deadly pathogens.

Who Can Use This Resource

  • Investigators in academia, not-for-profit organizations, industry, and government studying biodefense and emerging infectious diseases may request the use of biocontainment laboratories.

How To Get Started

  • Please contact the LIDR directly for further information.

Support Services

Laboratory and Analytical Support

  • Immunology services: Flow cytometry, cell sorting, ELISA, ELISPOT, post-mortem cytokine/chemokine analysis on high-throughput Luminex platform.
  • Pathology services: Necropsy, gross pathology, histology, immunohistochemistry, muscle, renal, liver and serum clinical chemistry profiles over seen by board certified laboratory animal and veterinary pathology veterinarians.
  • Microbiology services: Growth and titration of Yersinia pestis, Coxiella burnetti, Bacillus anthracis, Francisella tularensis, Brucella spp, Orientia tsutsugamushi, Sindbis virus, Dengue virus, Chickungunya virus. Post-mortem analysis of pathogens in tissues, including culture and real time PCR. DNA manipulation, PCR, and other molecular biology capabilities.
  • Cell Culture: tissue- and cell-culture capabilities.

Ability To Accommodate cGLP or cGMP Studies?

No.

Tools

Animal Models, Including Species, Disease, and Delivery Methods

  • Mice: pneumonic, bubonic and septicemic plague, aerosol and flea transmission models.
  • Q fever, i.p. model; tularemia, oral, i.n., i.p., i.t.; brucellosis, oral, i.n. and i.p. models; scrub typhus, i.p model.
  • Guinea pig: Q fever i.p. and aerosol transmission models.
  • Mosquitos: Chickungunya, oral, i.p.; Dengue, oral i.p.; Sindbis, oral, i.p.

Pathogens

  • Yersinia pestis
  • Coxiella burnetti
  • Bacillus anthracis
  • Francisella tularensis
  • Brucella abortus, suis and melitensis
  • Burkholderia pseudomaelli
  • Orientia tsutsugamushi
  • Coccidioides immitis
  • Sindbis virus
  • Dengue virus
  • Chickungunya virus
  • Heartland virus
  • Botulin toxin producing strain of Clostridium botulinum
  • T2 toxin

Imaging Capabilities

  • Olympus and Nikon Eclipse fluorescent microscopes

Major Equipment Available

All operational and available in a BSL3/ABSL3 environment.

  • Three laser MoFLo XDP cell sorter
  • CH technologies nose only inhalation exposure system
  • Untracentrifuge
  • PCR services
  • Electroporators
  • Thermocycler, cell- and tissue-culture incubators

Shared Resources

  • Fortessa, CyAn ADP, FACScan, FACSCalibur, flow cytometers and MoFlo XDP cell sorter
  • High throughput DNA and metagenomics sequencing, proteomic, metabolomic, X ray crystallography and transgenic rodent production services
  • Transmission and scanning electron microscopes
  • Laser scanning confocal microscopes
  • Laser capture microdissection systems
  • Leica DMI4000B inverted microscope and integrated Eppendorf microinjection system
  • 600 and 800 MHz spectrometers for NMR studies, transgenic pig production services
  • Canine Genetic Disease Testing Laboratory
Content last reviewed on January 12, 2018