Flow Cytometry Section
Iyadh Douagi, Ph.D.
Chief, Flow Cytometry Section
Major Areas of Research
- High-dimensional single cell analysis and cell sorting (BSL2, BSL2 Enhanced and BSL3)
- Multispectral imaging cytometry
- Multiplex bead array analysis
- Custom antibodies and proteins conjugation
Program Description
The Flow Cytometry Section (FCS) provides DIR investigators with cutting-edge cytometric technologies and instrumentation for high dimensional cell analysis and sorting. In addition, the FCS provides training, consultation, method development, and support for experiments involving broad aspects of cytometry applications. Major goals within the FCS are to provide access to state-of-the-art technologies, to help design and run experiments, to facilitate data interpretation, and to provide results that are of consistent high quality. This mission is accomplished through the efforts of staff with extensive cytometry experience.
Biography
Iyadh Douagi, Ph.D. is Chief of the Flow Cytometry Section (FCS) at NIAID. FCS provides Division of Intramural Research investigators with cutting-edge cytometric technologies and instrumentation for high dimensional cell analysis and sorting.
Instrumentation
Main facility - NIH campus (Building 4)
Cell Sorters
- BD FACS Aria Fusion-Orange
- 18-color capability,
- 6 lasers: 355nm, 405nm, 445nm, 488nm, 561nm, and 640nm
- Located in Class II Biological Safety Cabinet
- BD FACS Aria III-Red cell sorter
- 17-color capability
- 4 lasers: 405nm, 488nm, 561nm, and 640nm
- Located in Class II Biological Safety Cabinet
- BD FACS Aria III-Blue cell sorter
- 15-color capability
- 4 lasers: 405nm, 488nm, 561nm, and 638nm
Analyzers
- BD LSR II
- 18-color capability
- 4 lasers: 355nm, 405nm, 488nm, and 640nm
- BD LSR Fortessa SORP
- 18-color capability
- 4 lasers: 405nm, 488nm, 532nm, and 640nm
- BD Symphony A5 with HTS
- 28-color capability
- 5 lasers: 355nm, 405nm, 488nm, 532nm, and 628nm
NIH campus (Building 50)
Analyzers
- BD FACS CANTO
- 8-color capability
- 3 lasers: 405nm, 488nm, and 640nm
- AMNIS ImagestreamX Mark II
- 4 Lasers, 10-color capability, dark field and brightfield
- The ImageStream combines the speed, sensitivity, and phenotyping abilities of flow cytometry with the detailed imagery and functional insights of microscopy.
- Millipore MagPix
- Up to 50 analytes/sample
- The MagPix analyzer is based on the principles of flow cytometry. Using very small sample volumes, it enables to multiplex up to 50 analytes in a single microplate well. The system delivers fast and cost-effective bioassay results on many assay formats including nucleic acid assays, receptor-ligand assays, immunoassays and enzymatic assays.
NIH campus (Building 33)
Cell Sorters – BSL2
- BSL2 FACS Aria II-Emerald Special Order cell sorter
- 16-color capability
- 4 lasers: 405nm, 488n, 532nm, and 640nm
- BSL2 FACS Aria II-Cyan Special Order cell sorter
- 13-color capability
- 4 lasers: 405nm, 488nm, 561nm, and 640nm
Cell sorters – BSL3
With the increase in demand for high-speed cell sorting of viable infectious samples in the DIR, it is important for NIAID to have infectious cell sorting capabilities in BSL-3 laboratories. There are only a handful of BSL3 cell sorters worldwide, so the DIR is fortunate to have the instrumentation and expertise on campus.
- BSL3 FACS Aria II-Silver cell sorter (select and non-select agent BSL3 pathogens)
- 16-color capability
- 4 lasers: 405nm, 488nm, 561nm and 640nm
Analyzers
- BD Symphony A5 with HTS
- 28-color capability
- 5 lasers: 355nm, 405nm, 488nm, 532nm, and 628nm
- BSL3 BD LSR II with HTS
- 12-color capability
- 3 lasers: 405nm, 488nm, and 640nm
Specialties
Through the custom Antibodies Services Facility, the FCS also provides intramural NIAID investigators, cost- and time-efficient methods of obtaining fluorescent-labeled antibodies. Resources include: Hybridoma expansion, antibody purification from ascites, antisera, and hybridoma supernatant, and coupling of purified antibodies and proteins to various fluorochromes.
Custom Antibodies Services Facility Contact: Larry M. Lantz, Ph.D.
Research Group
- Iyadh Douagi, Ph.D.
- Dareskedar Admassu, M.S.
- Cohen Melanie, M.S.
- Calvin Eigsti, B.A.
- Thomas Moyer, B.S.
- Dayton Nance, B.S.
- Carol Henry, B.S.
- Larry M. Lantz, Ph.D.
- David Stephany, B.S.