Notice

Because of a lapse in government funding, the information on this website may not be up to date, transactions submitted via the website may not be processed, and the agency may not be able to respond to inquiries until appropriations are enacted.

Updates regarding government operating status and resumption of normal operations can be found at USA.gov.

NIAID Microbiome Program: Sequencing

NIAID’s microbiome sequencing facility studies the structure and function of the microbiome associated with various hosts and body sites. This facility has one dedicated staff member and is equipped with an Ilumina MiSeq, which can sequence whole genomes or specific amplicons. Whole genome sequencing is regularly used to characterize entire genomes from complex microbial communities or unique fungal or bacterial isolates in an unbiased manner. This approach allows the investigator to compare microbial communities and their gene content in different environments.

The facility caters to a variety of research collaborators undertaking small to large sequencing projects.

Main Areas of Focus

  • To allow investigators to compare microbial communities and their gene content in different environments
  • Using whole genome sequencing, to provide a broad idea of the potential functional capacity of the communities or single species sequenced
  • Using amplicon sequencing, to target the prokaryotic or fungal eukaryotic ribosomes of the different members of the microbiota for species identification and allow researchers to determine the structure of complex communities

Support Services

  • Whole genome sequencing
  • Expertise, infrastructure, tools, and training services for the NIAID Microbiome Project
  • Expertise in bioinformatics and computational biology methods focused on metadata collection for downstream analysis and cross-study comparisons, as well as approaches for data analysis

Tools

  • Access to computational resources and data storage for fast, efficient analysis and data retrieval
  • Data management and analysis tools have been baked into the BCBB-developed Nephele platform

How To Get Started

  • Before beginning a project, a project proposal must be submitted to an oversight committee to be reviewed for scientific rationale and feasibility. 
Content last reviewed on November 17, 2017