NIAID Microbiome Program: Sequencing

NIAID’s microbiome sequencing facility studies the structure and function of the microbiome associated with various hosts and body sites. This facility has one dedicated team and is equipped with an Ilumina MiSeq, which can sequence whole genomes or specific amplicons. Whole genome sequencing is regularly used to characterize entire genomes from complex microbial communities or unique fungal or bacterial isolates in an unbiased manner. This approach allows the investigator to compare microbial communities and their gene content in different environments.

The facility caters to a variety of research collaborators undertaking small to large sequencing projects.

Main Areas of Focus

  • To allow investigators to compare microbial communities and their gene content in different environments
  • Using amplicon sequencing, to target the prokaryotic or fungal eukaryotic ribosomes of the different members of the microbiota for species identification and allow researchers to determine the structure of complex communities
  • Using whole genome sequencing, to provide a broad idea of the potential functional capacity of the communities or single species sequenced

Support Services

  • 16s Amplicon sequencing
  • Metagenomic sequencing
  • Whole genome sequencing
  • RNA sequencing
  • Bioinformatics and computational biology analysis

Tools

  • Data management and analysis tools have been baked into the BCBB-developed Nephele platform

How To Get Started

  • Contact Xing He
  • You will then receive instructions and a Project Proposal Form
  • Submit Project Proposal Form
  • Schedule a project discussion meeting with the Microbiome Core
  • Start project        

NOTE:

  • To ensure optimal project design and results, it's highly recommended to first discuss it with Microbiome Core before beginning any project or experiment

Contact Information