SomaScan® V4.1

Credit: NIAID

SomaScan Assay profiles more than 7,000 proteins in only 55 µL of sample. The assay runs on Tecan’s Fluent liquid handling platform and utilizes a unique set of capture reagents called Slow Off rate Modified Aptamers (SOMAmers) to measure the relative abundance of proteins in a multitude of biological matrices including human serum, plasma, cerebrospinal fluid, urine as well as many others. Through a unique reagent binning design, the assay is capable of spanning 10 logs of dynamic range. The platform is high throughput and is capable of running 81subject samples at a time. In addition, it is highly reproducible, and the median %CV in replicate samples is typically <5%. It has proved successful in the identification of biomarker signatures in a variety of biomedical applications, especially in studies utilizing samples with paired timepoints to characterize responses to interventions and therapies.

Who Can Use This Resource

This technology is available to NIH researchers through the Center for Human Immunology, Inflammation, and Autoimmunity (CHI) Fee For Service Program. This program requires investigators to purchase the required SomaScan reagents that will be processed by NIAID SomaScan technicians.

How To Access

For inquiries contact Brian Sellers.

Location

The SomaScan V4.1 Assay is performed on the Tecan Fluent instrument that is located at the Center for Human Immunology, Inflammation, and Autoimmunity located in Building 10 on the Bethesda campus.

Research Network Affiliation

NIH Center for Human Immunology, Inflammation, and Autoimmunity

The NIH Center for Human Immunology, Inflammation, and Autoimmunity (CHI) is an affiliate of the National Institute of Allergy and Infectious Diseases Research Technologies Branch. CHI staff collaborate with researchers to apply advanced technologies to achieve an integrated and predictive understanding of human immunity, immune-microbiome behavior and their function in health and disease.

For inquiries about the Center for Human Immunology, Inflammation, and Autoimmunity contact:

Rachel Tracy
Executive Assistant

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