Immunology—Integrated Research Facility at Fort Detrick

The immunology laboratory at the Integrated Research Facility at Fort Detrick (IRF-Frederick) offers a variety of capabilities to interrogate the immune responses against pathogens requiring maximum containment. These include but are not limited to cytometry (e.g., immunophenotyping), Luminex multiplex protein/cytokine arrays, cartridge-based cell- sorting (MACSQuant Tyto), ELISAs, serology, in vitro functional assays, intracellular cytokine staining (ICS) enzyme-linked immune absorbent spot (ELISPOT; e.g., T cell and B cell).

Main Areas of Focus

  • Immunophenotyping and immune kinetics
  • Pathogen-specific serology
  • Multiplex cytokine assays and analysis
  • In vitro functional assays

Capabilities and Specialized Equipment

  • Custom pathogen-specific assay development
  • Pathogen-specific prescreening
  • Data interpretation and analysis
  • BD LSRFortessa flow cytometers with four lasers (405 nm, 488 nm, 561 nm, 640 nm) (two at BSL-4, two at BSL-2) and up to 16 fluorescent parameters
  • Luminex Flexmap 3D Instrument System (one at BSL-4)
  • Cytek Aurora flow cytometer with five lasers (355 nm, 405 nm, 488 nm, 561 nm, 640 nm) (two planned to go into BSL-4, two currently at BSL-2) and up to 63 simultaneous fluorescent parameters
  • ONI Nanoimager S super-resolution microscope with four lasers (405 nm, 488 nm, 561 nm, 640 nm) for PALM, dSTORM, smFRET, and 3D single-particle tracking (one planned to go into BSL-4)
  • Miltenyi Biotec MACSQuant Tyto cartridge-based cell sorter with three lasers (405 nm, 488 nm, 638 nm) (one planned to go into BSL-4) (Low pressure: 3-psi cartridge, 18-psi high-speed cartridge) with up to eight simultaneous fluorescent parameters
  • Miltenyi Biotec gentleMACS Octo Dissociators with Heaters (two at BSL-4)
  • Tecan Infinite M200 PRO multimode plate reader (absorbance, fluorescence, luminescence)
  •  AquaMax 2000 plate washer with magnetic and cell-washing capabilities

Research Photos

Erika and Tony work in the biosafety cabinet within the BSL-4 laboratory to isolate and stain cells attained from tissues collected during necropsy; subsequently, the cells will be analyzed via flow cytometry.
Erika and Tony work in the biosafety cabinet within the BSL-4 laboratory to isolate and stain cells attained from tissues collected during necropsy; subsequently, the cells will be analyzed via flow cytometry.
Credit
NIAID IRF-Frederick

Erika and Tony work in the biosafety cabinet within the BSL-4 laboratory to isolate and stain cells attained from tissues collected during necropsy; subsequently, the cells will be analyzed via flow cytometry.

Credit: NIAID IRF-Frederick
Rebecca processes a stained and fixed whole-blood sample on the Cytek Aurora Flow Cytometer in the BSL-2 laboratory.
Rebecca processes a stained and fixed whole-blood sample on the Cytek Aurora Flow Cytometer in the BSL-2 laboratory.
Credit
NIAID IRF-Frederick

Rebecca processes a stained and fixed whole-blood sample on the Cytek Aurora Flow Cytometer in the BSL-2 laboratory.

Credit: NIAID IRF-Frederick
Tony prepares a chamber slide in the BSL-2 laboratory. These slides are used for imaging with the ONI Nanoimager S super-resolution microscope.
Tony prepares a chamber slide in the BSL-2 laboratory. These slides are used for imaging with the ONI Nanoimager S super-resolution microscope.
Credit
NIAID IRF-Frederick

Tony prepares a chamber slide in the BSL-2 laboratory. These slides are used for imaging with the ONI Nanoimager S super-resolution microscope.

Credit: NIAID IRF-Frederick

Location

Integrated Research Facility at Fort Detrick (IRF-Frederick)

Contact Information

Jens H. Kuhn, M.D., Ph.D., Ph.D., M.S.
Principal Scientist and Director of Virology (Contractor)

IRF-Frederick

Standards

All procedures are well-documented and adhere to standard operating procedures (SOPs), methods, or study-approved plans and agreements.

Collaboration Opportunities

  • Studies relevant to human disease
  • Use of surrogate systems to test clinical hypotheses
  • Use of biological systems to answer questions regarding disease pathogenesis and strategies for intervention including antimicrobials, vaccines, and other countermeasures
  • Developing and incorporating cutting-edge technologies to understand infectious diseases

Read more about how to work with the IRF-Frederick.