Immunology—Integrated Research Facility at Fort Detrick

The immunology laboratory at the Integrated Research Facility at Fort Detrick (IRF-Frederick) offers a variety of capabilities to interrogate the immune responses against pathogens requiring maximum containment. These include but are not limited to cytometry (e.g., immunophenotyping), Luminex (multiplex protein/cytokine arrays), cell purification (MACS - Miltenyi), ELISAs, serology, in vitro functional assays, polyfunctional T-cell assay (PFTA), intracellular cytokine staining (ICS), and ELISPOT (e.g., T and B cell).

Main Areas of Focus

  • Immunophenotyping and immune kinetics
  • Pathogen-specific serology
  • Multiplex cytokine assays and analysis
  • In vitro functional assays

Capabilities and Specialized Equipment

  • Custom pathogen-specific assay development
  • Pathogen-specific prescreening
  • Data interpretation and analysis
  • BD LSR Fortessa (BSL-4, 2 at BSL-2) with up to 18 simultaneous parameters
  • Luminex Flexmap 3D (1 at BSL-4, 1 at BSL-2)
  • Miltenyi Gentle MACS Octo Dissociators with Heaters (2 at BSL-4)
  • Tecan Infinite M200 Pro Plate Reader (Absorbance, Fluorescence, Luminescence)
  • AquaMax 2000 Plate Washer with magnetic and cell-washing capabilities

Standards

All procedures are well-documented and adhere to standard operating procedures (SOPs), methods, or study-approved plans and agreements.

Collaboration Opportunities

  • Studies relevant to human disease
  • Use of surrogate systems to test clinical hypotheses
  • Use of biological systems to answer questions regarding disease pathogenesis and strategies for intervention including antimicrobials, vaccines, and other countermeasures
  • Developing and incorporating cutting-edge technologies to understand infectious diseases

Read more about how to work with the IRF-Frederick.

Location

Integrated Research Facility at Fort Detrick (IRF-Frederick)

Contact Information

Jens H. Kuhn, M.D., Ph.D., Ph.D., M.S.
Principal Scientist and Director of Virology (Contractor)