STUDY DESCRIPTION:
The purpose of this study is to collect tonsil tissues that are routinely discarded after tonsillectomy procedures to develop lymphoid organoid models to evaluate host-pathogen interactions in human health and disease. One such interaction is the human immunotolerance mechanism to the Chemotaxis Inhibitory Protein of S. aureus (CHIPS).
OBJECTIVES:
Primary Objectives:
-Develop a lymphoid organoid model from discarded human tonsils and determine and compare the human immunologic signatures of primary exposure, within the antigenic sin contexts of re-exposure, and with repeated exposures to CHIPS.
Secondary Objective:
-Modulate anti-CHIPS IgG4 class switching through variation of primary and costimulatory signals.
ENDPOINTS:
Primary Endpoints:
Differences between the following within the antigenic sin contexts of re-exposure, and with repeated exposures to CHIPS:
* Immune cell composition.
* Anti-CHIPS antibody levels, including total and subclasses of IgG and their neutralizing capacity.
* Cytokine levels.
* Activation-induced cytidine deaminase (AID) levels.
* Single-cell inference of class switch recombination (sciCSR).
Secondary Endpoints:
Differences between the following modulation of primary and costimulatory signals:
* Immune cell composition.
* Anti-CHIPS antibody levels, including total and subclasses of IgG and their neutralizing capacity.
* Cytokine levels.
* AID levels.
* sciCSR
The purpose of this study is to collect tonsil tissues that are routinely discarded after tonsillectomy procedures to develop lymphoid organoid models to evaluate host-pathogen interactions in human health and disease. One such interaction is the human immunotolerance mechanism to the Chemotaxis Inhibitory Protein of S. aureus (CHIPS).
OBJECTIVES:
Primary Objectives:
-Develop a lymphoid organoid model from discarded human tonsils and determine and compare the human immunologic signatures of primary exposure, within the antigenic sin contexts of re-exposure, and with repeated exposures to CHIPS.
Secondary Objective:
-Modulate anti-CHIPS IgG4 class switching through variation of primary and costimulatory signals.
ENDPOINTS:
Primary Endpoints:
Differences between the following within the antigenic sin contexts of re-exposure, and with repeated exposures to CHIPS:
* Immune cell composition.
* Anti-CHIPS antibody levels, including total and subclasses of IgG and their neutralizing capacity.
* Cytokine levels.
* Activation-induced cytidine deaminase (AID) levels.
* Single-cell inference of class switch recombination (sciCSR).
Secondary Endpoints:
Differences between the following modulation of primary and costimulatory signals:
* Immune cell composition.
* Anti-CHIPS antibody levels, including total and subclasses of IgG and their neutralizing capacity.
* Cytokine levels.
* AID levels.
* sciCSR
Visit ClinicalTrials.gov for details.