Shaden Kamhawi, Ph.D.

Vector Molecular Biology Section

Rockville, MD

Shaden Kamhawi, Ph.D. (She/Her/Hers)

Leishmaniasis Group Leader, Vector Molecular Biology Section

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Photo of Shaden Kamhawi, Ph.D.

Major Areas of Research

  • The host immune response to Leishmania transmission by sand fly bites
  • Development of Leishmania vaccines and markers of exposure to vector sand flies
  • Leishmania-sand fly interactions
  • Field-oriented investigations of the epidemiology of leishmaniasis and transmission patterns in cutaneous and visceral leishmaniasis foci

Program Description

The research program is focused on basic and translational aspects of leishmaniasis, a neglected vector-borne disease (NTD) transmitted by phlebotomine sand flies.

For basic research, the team uses Leishmania-infected sand flies as a natural transmission model for the study of leishmaniasis. Findings from the work emphasized the critical contribution of vectors in the initiation and establishment of vector-borne disease. Recently, the group demonstrated that vector-derived factors lead to acute inflammation found to be key to Leishmania parasite establishment and dissemination. The group also showed that bleeding at the site of bites, that is prolonged due to the anti-hemostatic effects of saliva, triggers the cell stress response and heme oxygenase-1 induction, which dampens inflammation. These bite-specific immune events shape the early response to Leishmania parasites and govern disease outcome. The group continues to investigate the host immune response to vector bites, using it in combination to known risk factors for disease development. Currently, in collaboration with Dr. Peter Melby, University of Texas Medical Department at Galveston, the group is investigating the effect of malnutrition on the course of sand fly transmitted visceral leishmaniasis.

For preclinical research, the team is involved in investigating the efficacy of promising vaccines against leishmaniasis using infected sand flies as challenge. We are currently validating a live attenuated vaccine developed by Hira Nakhasi (US Food and Drug Administration), Abhay Satoskar (Ohio State University) and Greg Matlashewski (McGill University), and working towards developing a human challenge model of cutaneous leishmaniasis.

For translational research, Dr. Kamhawi has a keen interest in field-oriented studies based in leishmaniasis foci. The group has made fundamental discoveries of factors controlling vector competence under laboratory conditions that have yet to be demonstrated in field settings. Currently, Dr. Kamhawi is collaborating with multiple international partners in the Indian subcontinent, East Africa, West Africa and Brazil looking at drivers of transmission in cutaneous and visceral leishmaniasis foci and assessing the relevance of sand fly gut microbiota and longevity in disease prevalence and pathogenicity. The group is also developing markers of exposure to specific vectors and will assess their efficacy as a surveillance tool to be used in monitoring human-vector contact in endemic populations during interventions. Main collaborators are Caryn Bern (UCSF), Sridhar Srikantiah (Care India), Abhay Satoskar (OSU), Seydou Doumbia (University of Bamako), Damaris Matoke (KEMRI), Asrat Hailu, (Addis Ababa University), Ahmed Musa (University of Khartoum) and Claudia Brodskyn (FIOCRUZ).

Dr. Kamhawi is a strong advocate for promoting science in NTD countries and has been serving the community as co-editor-in-chief for PLOS Neglected Tropical Diseases since 2019.

A tridimensional view of a macrophage (IBA-1, yellow; DAPI, blue) from mouse skin expressing heme oxygenase-1 (HO-1, red) after ingesting a red blood cell (TER-119, white) 18 hours after sand fly bites.
A tridimensional view of a macrophage (IBA-1, yellow; DAPI, blue) from mouse skin expressing heme oxygenase-1 (HO-1, red) after ingesting a red blood cell (TER-119, white) 18 hours after sand fly bites.
Credit
NIAID

A tridimensional view of a macrophage (IBA-1, yellow; DAPI, blue) from mouse skin expressing heme oxygenase-1 (HO-1, red) after ingesting a red blood cell (TER-119, white) 18 hours after sand fly bites.

Credit: NIAID

Biography

Education

Ph.D., Medical Entomology, Salford Univeristy, Salford, England

Languages Spoken

French, Arabic

Originally from Jordan, Dr. Kamhawi received her Ph.D. in Medical Entomology at Salford University in England in 1990. She returned to Jordan as an Assistant Professor at Yarmouk University where she worked for several years on leishmaniasis and hydatid disease, with a focus on transmission and risk factors in field settings. After she became an Associate Professor at Yarmouk University, she took a sabbatical in 1997 as a Visiting Scientist at NIAID’s Laboratory of Parasitic Diseases and become a Staff Scientist in 2000. In 2006, Dr. Kamhawi moved to NIAID’s Laboratory of Malaria and Vector Research (LMVR) as a Core Staff Scientist and was awarded the honorary title of Core Associate Scientist in 2014. Dr. Kamhawi is currently the Leishmaniasis Group Leader in the Vector Molecular Biology Section (VMBS). 

Selected Publications

Naomi E. Aronson, Fabiano Oliveira, Regis Gomes, William D. Porter, Robin S. Howard, Shaden Kamhawi and Jesus G. Valenzuela. Antibody Responses to Phlebotomus papatasi Saliva in American Soldiers With Cutaneous Leishmaniasis Versus Controls. Front. Trop. Dis, 2022.

Serafim TD, Coutinho-Abreu IV, Dey R, Kissinger R, Valenzuela JG, Oliveira F, Kamhawi S. Leishmaniasis: the act of transmission. Trends Parasitol. 2021 Nov;37(11):976-987. 

DeSouza-Vieira T, Iniguez E, Serafim TD, de Castro W, Karmakar S, Disotuar MM, Cecilio P, Lacsina JR, Meneses C, Nagata BM, Cardoso S, Sonenshine DE, Moore IN, Borges VM, Dey R, Soares MP, Nakhasi HL, Oliveira F, Valenzuela JG, Kamhawi S. Heme Oxygenase-1 Induction by Blood-Feeding Arthropods Controls Skin Inflammation and Promotes Disease Tolerance. Cell Rep. 2020 Oct;33(4):108317. 

Hotez PJ, Aksoy S, Brindley PJ, Kamhawi S. What constitutes a neglected tropical disease? PLoS Negl Trop Dis. 2020 Jan;14(1):e0008001. 

Serafim TD, Coutinho-Abreu IV, Oliveira F, Meneses C, Kamhawi S, Valenzuela JG. Sequential blood meals promote Leishmania replication and reverse metacyclogenesis augmenting vector infectivity. Nat Microbiol. 2018 May;3(5):548-555. 

Dey R, Joshi AB, Oliveira F, Pereira L, Guimarães-Costa AB, Serafim TD, de Castro W, Coutinho-Abreu IV, Bhattacharya P, Townsend S, Aslan H, Perkins A, Karmakar S, Ismail N, Karetnick M, Meneses C, Duncan R, Nakhasi HL, Valenzuela JG, Kamhawi S. Gut Microbes Egested during Bites of Infected Sand Flies Augment Severity of Leishmaniasis via Inflammasome-Derived IL-1β. Cell Host Microbe. 2018 Jan;23(1):134-143.e6.  
 

Visit PubMed for a complete publication listing.

Patents

Fischer L, Kamhawi S, Valenzuela J, Suau HA, inventors; The United States of America as represented by the Secretary of the Department of Health and Human Services, assignee. Leishmania challenge model. United States patent US 8,906,358. 9 December 2014.

Valenzuela JG, Belkaid Y, Kamhawi S, Sacks D, Ribeiro JMC, inventors; The United States of America as represented by the Secretary of the Department of Health and Human Services, assignee. Anti-arthropod vector vaccines, methods of selecting and uses thereof. United States patent US 7,964,576. 21 June 2011. 

Valenzuela JG, Ribeiro JMC, Kamhawi S, Belkaid Y, Fischer L, Audonnet JC, Milward F, inventors; The United States of America as represented by the Department of Health and Human Services, Merial Limited, assignees. P. ariasi polypeptides, p. perniciosuspolypeptides and methods of use. United States patent US 7,741,437. 22 Jun 2010.
Valenzuela JG, Belkaid Y, Kamhawi S, Sacks D, Ribeiro JMC, inventors; The United States of America as represented by the Department of Health and Human Services, assignee. Anti-arthropod vector vaccines, methods of selecting and uses thereof. United States patent US 7,388,089. 17 Jun 2008. 

Participating Research Networks

USA

  • The University of Texas Medical Branch, Galveston
  • University of California, San Francisco
  • Ohio State University
  • U.S. Food and Drug Administration
  • McGill University, Canada
  • Uniformed Services University of the Health Sciences
  • Oregon Health & Science University
  • University of Maryland
  • Yale School of Public Health
  • University of Iowa

International

  • Care India, India
  • University of Bamako, Mali
  • Addis Ababa University, Ethiopia
  • Kenya Medical Research Institute, Kenya
  • University of Khartoum, Sudan
  • CPqGM – FIOCRUZ, Brazil
  • Pasteur Institute of Tunis, Tunisia
     
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