NIH Center for Human Immunology, Inflammation, and Autoimmunity Technology

Major platforms and equipment currently maintained at CHI include:

Sequencing-based technology

  • 10x Genomics Chromium X for single cell sequencing library preparation, including CITE-seq and ASAP-seq to assess chromatin accessibility, transcriptome, and more than 100 surface proteins in the same single cells
  • Automated pipeline for NGS library preparation, including a Qiasymphony and BioMek for bulk RNA-seq
  • Illumina NextSeq 2000 for sequencing
  • Sony MA900 flow cytometer for cell sorting to enrich rare populations for sequencing

High-dimensional cytometry

  • Flow phenotyping with 30-40c panels for PBMC, T cell subsets, and specific tissues
  • Stimulation assays to simultaneously assess 8 phospho-protein signaling pathways in 20 PBMC populations
  • Cytek Aurora and BD FACSymphony for high parameter flow cytometry
  • Fluidigm Helios for mass cytometry

Aptamer based proteomics

  • SomaLogic run in-house to assay 7,000 protein analytes in peripheral blood serum/plasma
  • Exploratory application to other sample types such as CSF, urine, and saliva
  • Tecan Fluent 780 for automated assay performance
Content last reviewed on