Major platforms and equipment currently maintained at CHI include:
Sequencing-based technology
- 10x Genomics Chromium X for single cell sequencing library preparation, including CITE-seq and ASAP-seq to assess chromatin accessibility, transcriptome, and more than 100 surface proteins in the same single cells
- Automated pipeline for NGS library preparation, including a Qiasymphony and BioMek for bulk RNA-seq
- Illumina NextSeq 2000 for sequencing
- Sony MA900 flow cytometer for cell sorting to enrich rare populations for sequencing
High-dimensional cytometry
- Flow phenotyping with 30-40c panels for PBMC, T cell subsets, and specific tissues
- Stimulation assays to simultaneously assess 8 phospho-protein signaling pathways in 20 PBMC populations
- Cytek Aurora and BD FACSymphony for high parameter flow cytometry
- Fluidigm Helios for mass cytometry
Aptamer based proteomics
- SomaLogic run in-house to assay 7,000 protein analytes in peripheral blood serum/plasma
- Exploratory application to other sample types such as CSF, urine, and saliva
- Tecan Fluent 780 for automated assay performance