The Research Technologies Branch (RTB) offers NIAID researchers at the National Institutes of Health (NIH) campus in Bethesda, Maryland and at the Rocky Mountain Laboratories (RML) in Hamilton, Montana access to a variety of cytometry collaborative technologies to support their research.
Major Areas of Support
- Cell analysis and cell sorting including RG2, RG3 & RG4 agents
- Single cell sorting and cloning
- Ultra-deep immunophenotyping (30+ parameters)
- Spectral and Imaging-based cytometry
- Interface with other omics
- Custom antibodies services
- Consultation & training
3d render of a cytometry laser on a cell
Instrumentation
Analyzers - BSL2
- BD Symphony A5, HTS
- BD LSR Fortessa SORP
- Sony ID7000 Spectral Cell Analyzer
- BD LSR II
- AMNIS ImagestreamX Mark II
- Millipore MagPix
- BD Symphony A5, HTS (RML campus)
Analyzers - BSL3
- CytoFLEX LX in class II BSC, HTS
- CytoFLEX LX, HTS (RML campus)
Analyzers – BSL4
- CytoFLEX LX, HTS (RML campus)
Cell Sorting – BSL2/2+
- BSL2+ BD FACS Aria Fusion-Orange
- BSL2+ BD FACSymphony S6 Red
- BSL2+ FACS Aria III-Violet Special Order
- Cytek CS; spectral sorter
- BSL2+ FACS Aria (RML campus)
- BSL2+ BD FACSDiscover S8; image-based spectral sorter
Cell Sorting – BSL3
- BSL3 FACS Aria II-Silver
Selected Publications
R406 reduces lipopolysaccharide-induced neutrophil activation.
Warner S, Teague HL, Ramos-Benitez MJ, Panicker S, Allen K, Gaihre S, Moyer T, Parachalil Gopalan B, Douagi I, Shet A, Kanthi Y, Suffredini AF, Chertow DS, Strich JR. Cell Immunol. 2024 Jul 26;403-404:104860. doi: 10.1016/j.cellimm.2024.104860. Epub ahead of print. PMID: 39084187.
Peripheral apoptosis and limited clonal deletion during physiologic murine B lymphocyte development.
Simpson MJ, Newen AM, McNees C, Sharma S, Pfannenstiel D, Moyer T, Stephany D, Douagi I, Wang Q, Mayer CT. Nat Commun. 2024 Jun 1;15(1):4691. doi: 10.1038/s41467-024-49062-x. PMID: 38824171; PMCID: PMC11144239.
Cytometry in High-Containment Laboratories.
Cohen M, Laux J, Douagi I. Methods Mol Biol. 2024;2779:425-456. doi: 10.1007/978-1-0716-3738-8_20. PMID: 38526798.
Conjugation of Fluorochromes to Monoclonal Antibodies.
Lantz L, Holmes K, Douagi I. Curr Protoc. 2023 Jun;3(6):e795. doi: 10.1002/cpz1.795. PMID: 37358184; PMCID: PMC10369850.
Broadly neutralizing antibodies target the coronavirus fusion peptide.
Dacon C, Tucker C, Peng L, Lee CD, Lin TH, Yuan M, Cong Y, Wang L, Purser L, Williams JK, Pyo CW, Kosik I, Hu Z, Zhao M, Mohan D, Cooper AJR, Peterson M, Skinner J, Dixit S, Kollins E, Huzella L, Perry D, Byrum R, Lembirik S, Drawbaugh D, Eaton B, Zhang Y, Yang ES, Chen M, Leung K, Weinberg RS, Pegu A, Geraghty DE, Davidson E, Douagi I, Moir S, Yewdell JW, Schmaljohn C, Crompton PD, Holbrook MR, Nemazee D, Mascola JR, Wilson IA, Tan J. Science. 2022 Aug 12;377(6607):728-735. doi: 10.1126/science.abq3773. Epub 2022 Jul 12. PMID: 35857439; PMCID: PMC9348754.
Bispecific antibodies targeting distinct regions of the spike protein potently neutralize SARS-CoV-2 variants of concern.
Cho H, Gonzales-Wartz KK, Huang D, Yuan M, Peterson M, Liang J, Beutler N, Torres JL, Cong Y, Postnikova E, Bangaru S, Talana CA, Shi W, Yang ES, Zhang Y, Leung K, Wang L, Peng L, Skinner J, Li S, Wu NC, Liu H, Dacon C, Moyer T, Cohen M, Zhao M, Lee FE, Weinberg RS, Douagi I, Gross R, Schmaljohn C, Pegu A, Mascola JR, Holbrook M, Nemazee D, Rogers TF, Ward AB, Wilson IA, Crompton PD, Tan J. Sci Transl Med. 2021 Oct 20;13(616):eabj5413. doi: 10.1126/scitranslmed.abj5413. Epub 2021 Oct 20. PMID:34519517; PMCID: PMC8651051.
Risk awareness during operation of analytical flow cytometers and implications throughout the COVID-19 pandemic.
Aspland A, Chew C, Douagi I, Galland T, Marvin J, Monts J, Nance D, Smith AL, Solga M. Cytometry A. 2021 Jan;99(1):81-89. doi: 10.1002/cyto.a.24282. Epub 2020 Dec 23. PMID: 34038035.
Biosafety during a pandemic: shared resource laboratories rise to the challenge.
Aspland AM, Douagi I, Filby A, Jellison ER, Martinez L, Shinko D, Smith AL, Tang VA, Thornton S.Cytometry A. 2021 Jan;99(1):68-80. doi: 10.1002/cyto.a.24280. Epub 2021 Jan 4. PMID: 33289290; PMCID: PMC7753791.
Access
Currently, collaborating with RTB is only available to federally funded institutions. Researchers may access all RTB support services by visiting RTB on Inside NIAID (this link is only available to NIAID lab scientists).
Leadership
Iyadh Douagi, Ph.D.,
Chief, Cytometry Section
Iyadh Douagi, Ph.D.