PCHPI—Pittsburgh Center for HIV Protein Interactions

X-ray crystal structure of HIV-1 Vpr (red) in complex with uracil DNA glycosylase 2 (UNG2, green) and the Cul4-DCAF1 E3 ligase, comprising DDB1- and CUL4-associated factor 1 (DCAF1, blue), damage-specific DNA binding protein 1 (DDB1, orange), and Cullin4 (CUL4, gray).

Credit: PCHPI

Principal investigator: Angela Gronenborn, Ph.D., University of Pittsburgh School of Medicine

The PCHPI is focused on structure determination and imaging of pivotal events that occur during viral particle maturation, post-entry events, nuclear entry and integration. The center applies proteomic approaches to identify novel HIV-interacting host proteins and integrates virological and biochemical approaches in the interrogation of these interactions with structural methodologies for understanding the viral-host interplay. Structural methodologies encompass solution and solid-state NMR spectroscopy, X-ray crystallography, cryo-electron microscopy, and MD simulations, with an emphasis on further developing novel approaches that advance the capabilities of these techniques.

Visit the PCHPI Center Website.

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